PMID- 1317386
OWN - NLM
STAT- MEDLINE
DCOM- 19920629
LR  - 20181130
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 74
IP  - 6
DP  - 1992 Jun
TI  - Interleukin-1 (IL-1)-induced IL-6- and IL-6-receptor-mediated release of human 
      chorionic gonadotropin by choriocarcinoma cell lines (Jar and HCCM-5) activates 
      adenosine 3',5'-monophosphate-independent signal transduction pathway.
PG  - 1389-95
AB  - The status of preservation of the ability to secrete cytokines, such as 
      interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF alpha), and IL-6, and the 
      cytokine-mediated regulatory cascade was investigated in four choriocarcinoma 
      cell lines. Each cell line constitutively produced IL-6, but not IL-1 alpha, IL-1 
      beta, or TNF alpha. Jar and HCCM-5 cells responded to IL-6, releasing hCG by 
      direct activation of IL-6 receptors (IL-6-R) with IL-6. Both cell lines also 
      responded to IL-1, but failed to responded to TNF alpha. When stimulated with 
      recombinant IL-1 alpha, both cell lines released IL-6 and activated the IL-6-R 
      system to release hCG, whereas stimulation with TNF alpha failed to release hCG. 
      The experiments showed that both the Jar and HCCM-5 cell lines possessed a 
      partially intact cytokine-mediated cascade, suggesting that IL-1-induced IL-6 
      release and IL-6-R activation operate in an autocrine manner. In contrast, NUC-1 
      and SCH cells failed to respond to IL-6, IL-1, or TNF alpha. Although 
      8-bromo-cAMP, which is a cAMP analog, stimulates hCG release by Jar cells, it 
      failed to stimulate IL-6 release. Moreover, cAMP-mediated hCG release was not 
      blocked by PM1, an anti-IL-6-R antibody. This suggests that elevation of the 
      cytoplasmic cAMP level might activate a pathway different from the IL-6- and 
      IL-6-R-dependent pathway. Moreover, IL-1- and IL-6-mediated hCG release was not 
      blocked by H8, a cAMP-dependent kinase inhibitor, which further suggests that the 
      IL-1- and IL-6-mediated pathway functions independently of the cAMP-dependent 
      pathway in releasing hCG in Jar cells.
FAU - Taniguchi, T
AU  - Taniguchi T
AD  - Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
FAU - Matsuzaki, N
AU  - Matsuzaki N
FAU - Jo, T
AU  - Jo T
FAU - Saji, F
AU  - Saji F
FAU - Taga, T
AU  - Taga T
FAU - Hirano, T
AU  - Hirano T
FAU - Kishimoto, T
AU  - Kishimoto T
FAU - Tanizawa, O
AU  - Tanizawa O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Isoquinolines)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interleukin-6)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - 84478-11-5 (N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/*pharmacology
MH  - Cell Line
MH  - Choriocarcinoma
MH  - Chorionic Gonadotropin/*metabolism
MH  - Cyclic AMP/metabolism/*pharmacology
MH  - Female
MH  - Humans
MH  - Interleukin-1/metabolism/*pharmacology
MH  - Interleukin-6/biosynthesis/*pharmacology/physiology
MH  - Isoquinolines/pharmacology
MH  - Kinetics
MH  - Pregnancy
MH  - Protein Kinase Inhibitors
MH  - Receptors, Immunologic/drug effects/*physiology
MH  - Receptors, Interleukin-6
MH  - Recombinant Proteins/pharmacology
MH  - Signal Transduction/*physiology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Uterine Neoplasms
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
AID - 10.1210/jcem.74.6.1317386 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1992 Jun;74(6):1389-95. doi: 10.1210/jcem.74.6.1317386.