PMID- 1318071
OWN - NLM
STAT- MEDLINE
DCOM- 19920713
LR  - 20191021
IS  - 0923-1811 (Print)
IS  - 0923-1811 (Linking)
VI  - 3
IP  - 2
DP  - 1992 Mar
TI  - Tumor necrosis factor-alpha (TNF-alpha), interferon-alpha (IFN-alpha) and 
      interferon-gamma (IFN-gamma) receptors on human normal and scleroderma dermal 
      fibroblasts in vitro.
PG  - 82-90
AB  - Interferons alpha and gamma (IFN-alpha, IFN-gamma) and tumor necrosis factor 
      alpha (TNF-alpha) exert different regulatory effects on the proliferation and 
      biosynthetic activities of human dermal fibroblasts. Inasmuch as these cytokines 
      bind to specific receptors in order to exert their activities, the expression of 
      IFN-alpha, IFN-gamma and TNF-alpha receptors on fibroblasts from human adult 
      normal and scleroderma skin cultured in vitro were quantitated. Adsorption was 
      detected by incubating confluent normal and scleroderma fibroblasts with various 
      concentrations of [125I]cytokine. Replicate experiments revealed 19,742 +/- 2057 
      (Kd = 1.15 x 10(-9) M) TNF-alpha receptors per normal dermal fibroblast and 
      15,006 +/- 75 (Kd = 6.75 x 10(-10) M) TNF-alpha receptors per scleroderma 
      fibroblast. Cross-linking 125I-TNF-alpha to its receptor on normal and 
      scleroderma fibroblasts revealed 130- and 100-kDa TNF-receptor complexes. 
      Although no quantitative or qualitative differences were detected between these 
      two cell types with regard to receptor numbers, TNF-alpha affinity or receptor 
      protein as detected by radiolabelled TNF-alpha, differences were detected in 
      levels of mRNA specific for TNF-alpha receptors. Northern blot analysis revealed 
      normal fibroblasts to constitutively contain mainly mRNA specific for the 55-kDa 
      TNF receptor and indicate that they are capable of responding to 
      TNF-alpha-induced up-regulation of mRNA specific for the 75 kDa TNF receptor. 
      Scleroderma fibroblasts, however, constitutively contain mRNA for both TNF 
      receptors and fail to respond to TNF-alpha up-regulation of the message for the 
      75-kDa receptor for TNF.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Berman, B
AU  - Berman B
AD  - Dermatology Department, University of California, Davis School of Medicine.
FAU - Wietzerbin, J
AU  - Wietzerbin J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (Cytokines)
RN  - 0 (Interferon-alpha)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interferon)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cytokines/*metabolism
MH  - Fibroblasts/immunology/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Interferon-alpha/metabolism
MH  - Interferon-gamma/metabolism
MH  - Receptors, Cell Surface/*metabolism
MH  - Receptors, Immunologic/metabolism
MH  - Receptors, Interferon
MH  - Receptors, Tumor Necrosis Factor
MH  - Scleroderma, Localized/immunology/*metabolism
MH  - Skin/immunology/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
AID - 0923-1811(92)90040-I [pii]
AID - 10.1016/0923-1811(92)90040-i [doi]
PST - ppublish
SO  - J Dermatol Sci. 1992 Mar;3(2):82-90. doi: 10.1016/0923-1811(92)90040-i.