PMID- 1318324
OWN - NLM
STAT- MEDLINE
DCOM- 19920713
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 89
IP  - 6
DP  - 1992 Jun
TI  - Infection of peripheral blood mononuclear cells by herpes simplex and 
      Epstein-Barr viruses. Differential induction of interleukin 6 and tumor necrosis 
      factor-alpha.
PG  - 1849-56
AB  - Infection by herpesviruses can result in profound immunosuppressive or 
      immunomodulatory effects. However, no significant information is available on the 
      effect of such infections on the production of immunoregulatory cytokines. We 
      studied the kinetics of production of two monocyte-derived cytokines, interleukin 
      6 (IL-6) and tumor necrosis factor-alpha (TNF alpha), induced by Epstein-Barr 
      virus (EBV) and herpes simplex virus type 1 (HSV-1) in peripheral blood 
      mononuclear cell cultures and in fractionated cell populations. We observed that, 
      when compared to HSV-1, EBV is a stronger inducer of IL-6. In EBV-infected 
      cultures, IL-6 protein was detected at day 1 postinfection and gradually 
      increased with time. In contrast, lower amounts of IL-6 were detected 5 d 
      postinfection in HSV-1-infected cultures. HSV-1-infected cultures secreted 
      significant amounts of TNF alpha protein after 5 d of culture and reached a 
      maximal level of production at day 7, whereas EBV inhibited TNF alpha production. 
      In fractionated cell populations, monocytic cells were found to be the main 
      source of IL-6 synthesis after EBV or HSV-1 infection. However, TNF alpha 
      synthesis in HSV-1-infected cultures was from both B and monocytic cells. By 
      using the polymerase chain reaction technique we show that, after infection by 
      these two herpesviruses, differences in cytokine gene products are also observed 
      at the transcriptional level. These observations demonstrate that EBV and HSV-1 
      exert differential effects on IL-6 and TNF alpha gene transcription and on the 
      resulting protein secretion in human mononuclear blood cells.
FAU - Gosselin, J
AU  - Gosselin J
AD  - Laboratory of Immunovirology, Faculty of Medicine, University of Montreal, 
      Quebec, Canada.
FAU - Flamand, L
AU  - Flamand L
FAU - D'Addario, M
AU  - D'Addario M
FAU - Hiscott, J
AU  - Hiscott J
FAU - Menezes, J
AU  - Menezes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Interleukin-6)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Herpesvirus 4, Human/*physiology
MH  - Humans
MH  - Interleukin-6/*biosynthesis/metabolism
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Monocytes/cytology/metabolism/*microbiology
MH  - Oligodeoxyribonucleotides
MH  - Polymerase Chain Reaction
MH  - Simplexvirus/*physiology
MH  - Transcription, Genetic
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/metabolism
MH  - Virus Replication
PMC - PMC295883
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
PMCR- 1992/06/01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
PHST- 1992/06/01 00:00 [pmc-release]
AID - 10.1172/JCI115789 [doi]
PST - ppublish
SO  - J Clin Invest. 1992 Jun;89(6):1849-56. doi: 10.1172/JCI115789.