PMID- 1318949
OWN - NLM
STAT- MEDLINE
DCOM- 19920723
LR  - 20071114
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 73 ( Pt 6)
DP  - 1992 Jun
TI  - Recombination between a herpes simplex virus type 1 vector deleted for immediate 
      early gene 3 and the infected cell genome.
PG  - 1553-8
AB  - We have used a vector derived from a herpes simplex virus type 1 (HSV-1) mutant 
      deleted for 3.6 kbp of the essential immediate early gene 3 to transduce the Tn5 
      neomycin phosphotransferase (neor) gene into rodent and primate cells in culture. 
      The transgene was flanked by genomic sequences from the human hprt gene. We 
      demonstrate in this study that sequences introduced by infection with the 
      replication-defective HSV vector can be stably inserted into the cell genome by 
      recombination. Both the efficiency of stable transduction, measured by the number 
      of neor-positive colonies, and the number and length of the transgene sequences 
      inserted into the cell genome were found to be a function of cell type. The 
      transduction efficiency appeared to be influenced, at least in part, by the 
      cytopathic potential of the replication-defective HSV-1 vector, which was more 
      pronounced in primate than in rodent cells.
FAU - Roemer, K
AU  - Roemer K
AD  - Center for Molecular Genetics, University of California, San Diego, La Jolla 
      92093.
FAU - Johnson, P A
AU  - Johnson PA
FAU - Friedmann, T
AU  - Friedmann T
LA  - eng
GR  - HD 20034/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (IE1 protein, Human herpesvirus 1)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.- (Phosphotransferases)
RN  - EC 2.7.1.95 (Kanamycin Kinase)
SB  - IM
GS  - neo<up>r</up>
MH  - Animals
MH  - Blotting, Southern
MH  - Cell Line
MH  - Defective Viruses/genetics
MH  - Drug Resistance/genetics
MH  - Humans
MH  - *Immediate-Early Proteins
MH  - Kanamycin Kinase
MH  - Mutation
MH  - Phosphotransferases/genetics
MH  - *Recombination, Genetic
MH  - Simplexvirus/enzymology/*genetics
MH  - Viral Proteins/*genetics
MH  - Virus Integration
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
AID - 10.1099/0022-1317-73-6-1553 [doi]
PST - ppublish
SO  - J Gen Virol. 1992 Jun;73 ( Pt 6):1553-8. doi: 10.1099/0022-1317-73-6-1553.