PMID- 1319300
OWN - NLM
STAT- MEDLINE
DCOM- 19920729
LR  - 20190902
IS  - 0732-8893 (Print)
IS  - 0732-8893 (Linking)
VI  - 15
IP  - 4
DP  - 1992 May-Jun
TI  - Evaluation of variables in immunofluorescence procedures for the detection of 
      antibodies against human herpesvirus 6 (HHV-6).
PG  - 313-20
AB  - Ten human sera were used to study different parameters, namely, methods of smear 
      preparation and fixation, and age of infected HSB-2 cells in order to optimize 
      indirect immunofluorescence assay (IFA) and anticomplement immunofluorescence 
      (ACIF) procedures to measure antibody levels against HHV-6. Results showed a 
      greater sensitivity of rapid smear drying and methanol fixation over conventional 
      acetone smear preparation. Cells harvested 6 days after infection and fixed with 
      methanol exhibited a sharper and more intense fluorescence. IFA titers were 
      higher than those obtained with ACIF, although the latter procedure enabled the 
      distinction between three fluorescent sites. Reactivity pattern of individual 
      sera against infected cells was variable and indicated that the human immune 
      response to HHV-6 is directed against different antigens. An easier 
      interpretation and a better definition of the fluorescence of HSB-2 cell line 
      infected with HHV-6 strain Dv is obtained with the following conditions: cells 
      should be harvested at 5-8 days after infection (at the giant cell stage of 
      infection), cell smears have to be dried quickly before fixation with methanol at 
      -20 degrees C, and finally, they should be stained by IFA.
FAU - Couillard, M
AU  - Couillard M
AD  - Department of Microbiology, Faculty of Medicine, Laval University, Sainte-Foy, 
      Quebec, Canada.
FAU - Joly, J R
AU  - Joly JR
FAU - Deschenes, L
AU  - Deschenes L
FAU - Richer, G
AU  - Richer G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Diagn Microbiol Infect Dis
JT  - Diagnostic microbiology and infectious disease
JID - 8305899
RN  - 0 (Antibodies, Viral)
RN  - 1364PS73AF (Acetone)
RN  - 9007-36-7 (Complement System Proteins)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Acetone
MH  - Antibodies, Viral/blood
MH  - Cell Line
MH  - Complement System Proteins/immunology
MH  - *Fluorescent Antibody Technique
MH  - Herpesvirus 6, Human/immunology/*ultrastructure
MH  - *Histocytological Preparation Techniques
MH  - Humans
MH  - Methanol
MH  - Sensitivity and Specificity
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
AID - 0732-8893(92)90017-N [pii]
AID - 10.1016/0732-8893(92)90017-n [doi]
PST - ppublish
SO  - Diagn Microbiol Infect Dis. 1992 May-Jun;15(4):313-20. doi: 
      10.1016/0732-8893(92)90017-n.