PMID- 1320114
OWN - NLM
STAT- MEDLINE
DCOM- 19920804
LR  - 20180330
IS  - 0022-3166 (Print)
IS  - 0022-3166 (Linking)
VI  - 122
IP  - 7
DP  - 1992 Jul
TI  - Short-chain fatty acid production and fiber degradation by human colonic 
      bacteria: effects of substrate and cell wall fractionation procedures.
PG  - 1508-20
AB  - Three dietary fiber sources (corn fiber, oat bran, wheat bran) were analyzed for 
      chemical composition and potential fermentation by human colonic bacteria in 
      vitro. Total dietary fiber (TDF) concentration of substrates was 64.3, 11.1 and 
      50.4 g/100 g dry matter for corn fiber, oat bran and wheat bran, respectively. 
      Original material (ORIG), TDF fractions and simulated (SIM) cell wall fractions 
      (produced by combining cellulose, hemicelluloses and pectic substances in 
      proportions they represented in the cell wall) from each substrate were fermented 
      in vitro for 6, 12, 18, 24 or 48 h using inoculum prepared from freshly voided 
      feces from each of three human volunteers. Substrate dry matter remaining after 
      48 h of fermentation was 87.8, 39.8 and 73.5% for TDF fractions of corn fiber, 
      oat bran and wheat bran, respectively. Disappearance of ORIG fractions was 
      considerably greater than that of TDF due to fermentation of nonfibrous material. 
      Disruption of cell wall structure during isolation of polysaccharide fractions 
      allowed for dramatically increased fermentability of SIM relative to TDF. 
      Averaged across all treatments, production of the short-chain fatty acids, 
      acetate, propionate and butyrate, occurred in the molar ratio 63:21:16; however, 
      profiles of short-chain fatty acids produced were influenced by both treatment 
      and inoculum source. Extent of substrate fermentation varied among inoculum 
      donors, implying that colonic microbial activities differ among individuals. 
      Potential colonic fermentability of fiber sources was influenced by substrate, 
      method of fiber preparation and inoculum source.
FAU - Bourquin, L D
AU  - Bourquin LD
AD  - Department of Animal Sciences, University of Illinois, Urbana 61801.
FAU - Titgemeyer, E C
AU  - Titgemeyer EC
FAU - Garleb, K A
AU  - Garleb KA
FAU - Fahey, G C Jr
AU  - Fahey GC Jr
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Dietary Fiber)
RN  - 0 (Fatty Acids, Volatile)
SB  - IM
MH  - Adult
MH  - Cell Wall/metabolism
MH  - Colon/*metabolism/microbiology
MH  - Dietary Fiber/*metabolism
MH  - Edible Grain
MH  - Fatty Acids, Volatile/*biosynthesis
MH  - Feces/microbiology
MH  - Fermentation
MH  - Humans
MH  - Male
MH  - Triticum
MH  - Zea mays
EDAT- 1992/07/11 19:15
MHDA- 2001/03/28 10:01
CRDT- 1992/07/11 19:15
PHST- 1992/07/11 19:15 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1992/07/11 19:15 [entrez]
AID - 10.1093/jn/122.7.1508 [doi]
PST - ppublish
SO  - J Nutr. 1992 Jul;122(7):1508-20. doi: 10.1093/jn/122.7.1508.