PMID- 1320954
OWN - NLM
STAT- MEDLINE
DCOM- 19920820
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 80
IP  - 2
DP  - 1992 Jul 15
TI  - Distribution and phenotype of Epstein-Barr virus-harboring cells in Hodgkin's 
      disease.
PG  - 484-91
AB  - Distribution and phenotype of Epstein-Barr virus (EBV)-harboring cells were 
      determined in Hodgkin's disease (HD) biopsies by in situ hybridization with 
      [35S]-labeled RNA probes specific for the small EBV-encoded nuclear RNAs, EBER1 
      and EBER2, in some instances preceded by immunohistology for CD20, CD30, CD45RO, 
      and CD68 antigens, the T-cell receptor beta-chain, and latent membrane antigen 
      (LMP) of EBV. Twenty-three of 46 HD cases displayed EBER transcripts in all 
      Hodgkin and Reed-Sternberg (H-RS) cells, and 18 of these cases showed LMP 
      expression exclusively in neoplastic cells. EBER+ small reactive cells were 
      present in 39 cases in low numbers, and in three cases in abundance. Thus, 
      presence of H-RS cells with or without LMP expression was not accompanied by an 
      unrestricted proliferation of reactive EBER+/LMP- lymphoid cells in the majority 
      of HD patients. Simultaneous in situ hybridization with [35S]-labeled 
      immunoglobulin light chain (IgLC) gene probes and nonisotopically labeled EBER 
      probe showed a phenotype of mature B lymphocytes and a polyclonal composition for 
      a large proportion of the EBER+ small cells. However, in contrast to noninfected 
      cells, CD20 expression was not detectable in many of these cells, which may 
      indicate downregulation of certain differentiation antigens in latently 
      EBV-infected small lymphoid cells in vivo.
FAU - Herbst, H
AU  - Herbst H
AD  - Institute of Pathology, Klinikum Steglitz, Free University of Berlin, Germany.
FAU - Steinbrecher, E
AU  - Steinbrecher E
FAU - Niedobitek, G
AU  - Niedobitek G
FAU - Young, L S
AU  - Young LS
FAU - Brooks, L
AU  - Brooks L
FAU - Muller-Lantzsch, N
AU  - Muller-Lantzsch N
FAU - Stein, H
AU  - Stein H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Viral)
RN  - 0 (Proteins)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Viral)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribosomal Proteins)
RN  - 135844-68-7 (RPL22 protein, human)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Antigens, Viral/analysis
MH  - Biopsy
MH  - Herpesvirus 4, Human/genetics/*isolation & purification
MH  - Hodgkin Disease/immunology/*microbiology/*pathology
MH  - Humans
MH  - Lymph Nodes/immunology/microbiology/pathology
MH  - Phenotype
MH  - Proteins/analysis/genetics
MH  - RNA Probes
MH  - RNA, Viral/analysis/genetics
MH  - *RNA-Binding Proteins
MH  - Reed-Sternberg Cells/immunology/microbiology/pathology
MH  - *Ribosomal Proteins
MH  - Transcription, Genetic
EDAT- 1992/07/15 00:00
MHDA- 1992/07/15 00:01
CRDT- 1992/07/15 00:00
PHST- 1992/07/15 00:00 [pubmed]
PHST- 1992/07/15 00:01 [medline]
PHST- 1992/07/15 00:00 [entrez]
AID - S0006-4971(20)70625-7 [pii]
PST - ppublish
SO  - Blood. 1992 Jul 15;80(2):484-91.