PMID- 1321148
OWN - NLM
STAT- MEDLINE
DCOM- 19920814
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 267
IP  - 20
DP  - 1992 Jul 15
TI  - Mouse mammary tumor virus gene expression is suppressed by oligomeric 
      ellagitannins, novel inhibitors of poly(ADP-ribose) glycohydrolase.
PG  - 14436-42
AB  - Oligomeric ellagitannins (nobotanins B, E, and K) were found to be potent 
      inhibitors of poly(ADP-ribose) glycohydrolase purified from mouse mammary tumor 
      34I cells. Kinetic analysis revealed that the inhibition of nobotanin B (dimer) 
      was competitive with respect to the substrate poly(ADP-ribose), whereas nobotanin 
      E (trimer) and nobotanin K (tetramer) exhibited mixed-type inhibition. These 
      results suggest that the dimeric structure of ellagitannin may have a functional 
      domain that competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase 
      molecule. To determine the inhibitory effects of oligomeric ellagitannins on 
      poly(ADP-ribose) glycohydrolase in vivo, we examined their effects on 
      de-poly(ADP-ribosyl)ation of some chromosomal proteins in intact 34I cells that 
      was induced by glucocorticoid treatment. Nobotanin B caused 
      concentration-dependent inhibition of glucocorticoid-induced 
      de-poly(ADP-ribosyl)ation of HMG 14 and 17 and histone H1 in intact 34I cells. 
      Interestingly, this inhibition was associated with suppression of the 
      glucocorticoid-sensitive mouse mammary tumor virus (MMTV) mRNA synthesis. In 
      contrast, nobotanin E and K had little inhibitory effect on either 
      de-poly(ADP-ribosyl)ation of these proteins or induction of MMTV transcription 
      after glucocorticoid treatment. Nobotanin B but not E and K was taken into 34I 
      cells. These results may suggest that the suppression of glucocorticoid-sensitive 
      MMTV transcription results from in vivo inhibition of poly(ADP-ribose) 
      glycohydrolase by nobotanin B. These results also indicate the importance of 
      de-poly(ADP-ribosyl)ation of HMG 14 and 17 and histone H1 in regulation of 
      transcription of the glucocorticoid-sensitive MMTV gene.
FAU - Tsai, Y J
AU  - Tsai YJ
AD  - Department of Pharmacology, Faculty of Medical Sciences, Taipei Medical College, 
      Taiwan, Republic of China.
FAU - Aoki, T
AU  - Aoki T
FAU - Maruta, H
AU  - Maruta H
FAU - Abe, H
AU  - Abe H
FAU - Sakagami, H
AU  - Sakagami H
FAU - Hatano, T
AU  - Hatano T
FAU - Okuda, T
AU  - Okuda T
FAU - Tanuma, S
AU  - Tanuma S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Hydrolyzable Tannins)
RN  - 0 (Tannins)
RN  - 104696-16-4 (nobotanin B)
RN  - 113866-94-7 (nobotanin E)
RN  - 119683-39-5 (nobotanin K)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
RN  - EC 3.2.1.143 (poly ADP-ribose glycohydrolase)
SB  - IM
MH  - Animals
MH  - Binding, Competitive
MH  - Cell Nucleus/enzymology
MH  - Gene Expression/drug effects
MH  - Gene Expression Regulation, Viral/drug effects
MH  - Genes, Viral/drug effects
MH  - Glycoside Hydrolases/*antagonists & inhibitors/isolation & purification
MH  - *Hydrolyzable Tannins
MH  - Kinetics
MH  - Mammary Neoplasms, Experimental
MH  - Mammary Tumor Virus, Mouse/drug effects/*genetics
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Molecular Structure
MH  - Structure-Activity Relationship
MH  - Tannins/*pharmacology
MH  - *Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1992/07/15 00:00
MHDA- 1992/07/15 00:01
CRDT- 1992/07/15 00:00
PHST- 1992/07/15 00:00 [pubmed]
PHST- 1992/07/15 00:01 [medline]
PHST- 1992/07/15 00:00 [entrez]
AID - S0021-9258(19)49731-7 [pii]
PST - ppublish
SO  - J Biol Chem. 1992 Jul 15;267(20):14436-42.