PMID- 1321714
OWN - NLM
STAT- MEDLINE
DCOM- 19920825
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 207
IP  - 2
DP  - 1992 Jul 15
TI  - Dependence of the adenovirus tripartite leader on the p220 subunit of eukaryotic 
      initiation factor 4F during in vitro translation. Effect of p220 cleavage by 
      foot-and-mouth-disease-virus L-protease on in vitro translation.
PG  - 471-7
AB  - The adenovirus tripartite leader (TPT) 5' untranslated region (5'UTR) allows 
      translation in poliovirus-infected cells, in which the p220 subunit of eukaryotic 
      initiation factor 4F is degraded. This p220-independent translation was 
      investigated by measuring in vitro translation in a reticulocyte lysate of a 
      reporter gene, chloramphenicol acetyltransferase, coupled to the TPT 5'UTR. The 
      p220 subunit was degraded by translation of a foot-and-mouth-disease L-protease 
      construct. Surprisingly, the TPT 5'UTR was dependent on intact p220, as are other 
      naturally capped mRNA species. Translation of encephalomyocarditis virus RNA was 
      p220 independent, as expected from its ability to support internal, 
      cap-independent initiation. In vitro protein-synthesis experiments with purified 
      initiation factors confirmed the dependence of TPT mRNA translation on eukaryotic 
      initiation factor 4F. The relationship between adenovirus TPT-5'UTR-directed 
      translation and poliovirus-induced host cell shut-off is discussed.
FAU - Thomas, A A
AU  - Thomas AA
AD  - Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
FAU - Scheper, G C
AU  - Scheper GC
FAU - Kleijn, M
AU  - Kleijn M
FAU - De Boer, M
AU  - De Boer M
FAU - Voorma, H O
AU  - Voorma HO
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Eukaryotic Initiation Factor-4F)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (RNA Caps)
RN  - 0 (RNA, Viral)
RN  - EC 3.4.- (Endopeptidases)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Aphthovirus/enzymology
MH  - Cell-Free System
MH  - Endopeptidases/metabolism
MH  - Eukaryotic Initiation Factor-4F
MH  - *Gene Expression Regulation, Viral
MH  - In Vitro Techniques
MH  - Macromolecular Substances
MH  - *Peptide Chain Initiation, Translational
MH  - Peptide Initiation Factors/*metabolism/ultrastructure
MH  - RNA Caps
MH  - RNA, Viral/*genetics
MH  - Rabbits
EDAT- 1992/07/15 00:00
MHDA- 1992/07/15 00:01
CRDT- 1992/07/15 00:00
PHST- 1992/07/15 00:00 [pubmed]
PHST- 1992/07/15 00:01 [medline]
PHST- 1992/07/15 00:00 [entrez]
AID - 10.1111/j.1432-1033.1992.tb17073.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1992 Jul 15;207(2):471-7. doi: 10.1111/j.1432-1033.1992.tb17073.x.