PMID- 1323634
OWN - NLM
STAT- MEDLINE
DCOM- 19920911
LR  - 20071115
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 120
IP  - 2
DP  - 1992 Aug
TI  - Effect of cell proliferation on H-ferritin receptor expression in human T 
      lymphoid (MOLT-4) cells.
PG  - 239-43
AB  - We have previously demonstrated the presence of receptors specific for human 
      recombinant H ferritin (HrHF) on human T lymphoid cells (MOLT-4), and changes in 
      receptor number and binding affinity with growth and cell cycling have now been 
      examined. Specific binding of HrHF was maximal in MOLT-4 cells harvested during 
      exponential growth with the cells in the DNA synthesis phase of the cell cycle. 
      Specific binding decreased progressively to the plateau phase of growth with the 
      cells in the resting phase of the cell cycle. Scatchard analysis of the 
      competitive binding data for HrHF demonstrated that this decrease in binding was 
      associated with a reduction in receptor number, from 42,140 per cell to 10,306 
      per cell. Receptor binding affinity increased only minimally over this period, 
      from 7.1 x 10(7) L/mol to 14.9 x 10(7) L/mol. These results indicate that growth- 
      and cell cycle-induced changes in H-ferritin receptor expression are primarily 
      associated with changes in receptor number rather than receptor binding affinity. 
      The present study demonstrates that the expression of this receptor is associated 
      with the proliferative status of the cell and suggests that the H-ferritin 
      receptor may mediate the putative regulatory role of H-ferritin.
FAU - Moss, D
AU  - Moss D
AD  - Liver Unit, Queensland Institute of Medical Research, Bancroft Centre, Herston, 
      Australia.
FAU - Powell, L W
AU  - Powell LW
FAU - Arosio, P
AU  - Arosio P
FAU - Halliday, J W
AU  - Halliday JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Iron-Binding Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (ferritin receptor)
RN  - 9007-73-2 (Ferritins)
SB  - IM
MH  - Cell Cycle/physiology
MH  - Cell Division/*physiology
MH  - Cell Line
MH  - *DNA Replication
MH  - Ferritins/*metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - *Iron-Binding Proteins
MH  - Kinetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma
MH  - Receptors, Cell Surface/*biosynthesis/metabolism
MH  - T-Lymphocytes
MH  - Time Factors
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
AID - 0022-2143(92)90131-4 [pii]
PST - ppublish
SO  - J Lab Clin Med. 1992 Aug;120(2):239-43.