PMID- 1323947
OWN - NLM
STAT- MEDLINE
DCOM- 19920917
LR  - 20061115
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 12
IP  - 4
DP  - 1992 Jul-Aug
TI  - Expression of transforming growth factor alpha mRNA in benign and malignant 
      tissues derived from gynecologic patients with various proliferative conditions.
PG  - 1115-20
AB  - Growth factors are polypeptides involved in the regulation of normal and 
      malignant cell growth. Transforming growth factor alpha (TGF alpha) is one of 
      such protein growth factors that plays an important role in the regulation of 
      mammalian cell growth. In this study, the expression of TGF alpha mRNA was 
      studied in tissue specimens obtained at the time of surgery from patients with 
      benign and malignant gynecologic proliferative conditions. To analyze TGF alpha 
      mRNA expression we utilized the highly sensitive technique denoted Message 
      Amplification Phenotyping which can detect mRNA in single cells. This technique 
      consists of isolating RNA, reverse transcription of total cellular RNA to produce 
      copy DNA, followed by enzymatic amplification of TGF alpha cDNA fragments using 
      specific TGF alpha primers and polymerase chain reaction. The results showed 
      significant levels to TGF alpha mRNA expression in vulvar (100% of the cases 
      positive), cervical (66% positive), and endometrial (66% positive) carcinomas. 
      Moreover, vulvar condylomas produced by human papilloma virus (HPV) showed the 
      highest levels of TGF alpha mRNA expression of all the pathological tissues 
      examined. In contrast, vulvar melanoma, fibrocystic disease of the breast, and 
      certain ovarian tumors showed undetectable TGF alpha mRNA expression. Normal 
      mesodermal tissues such as myometrium, abdominal rectus muscle, and fallopian 
      tubes were negative for TGF alpha mRNA expression. However, TGF alpha mRNA was 
      present in normal cervix and in normal endometrium. The results showed that TGF 
      alpha mRNA expression is frequently associated with various malignant tumors and 
      HPV-induced lesions of epithelial origin, suggesting that TGF alpha mRNA protein 
      product may be a contributory factor in the progression of these pathological 
      tissue alterations. Finally, TGF alpha mRNA expression was not restricted to 
      malignant cells, suggesting that the TGF alpha mRNA protein product may function 
      as a mitogen in the normal human epithelial tissues examined.
FAU - Xynos, F P
AU  - Xynos FP
AD  - Laboratory of Molecular Oncology, DVA Medical Center, St. Louis, MO 63106.
FAU - Klos, D J
AU  - Klos DJ
FAU - Hamilton, P D
AU  - Hamilton PD
FAU - Schuette, V
AU  - Schuette V
FAU - Fernandez-Pol, J A
AU  - Fernandez-Pol JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Actins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
SB  - IM
MH  - Actins/analysis
MH  - Adult
MH  - Aged
MH  - Carcinoma in Situ/*chemistry
MH  - Carcinoma, Squamous Cell/*chemistry
MH  - Condylomata Acuminata/*chemistry
MH  - Endometrial Neoplasms/chemistry
MH  - Female
MH  - Genital Neoplasms, Female/*chemistry
MH  - Humans
MH  - Middle Aged
MH  - Ovarian Neoplasms/chemistry
MH  - *Papillomaviridae
MH  - RNA, Messenger/*analysis
MH  - Transforming Growth Factor alpha/*analysis
MH  - *Tumor Virus Infections
MH  - Uterine Cervical Neoplasms/chemistry
MH  - Vulvar Neoplasms/chemistry
EDAT- 1992/07/01 00:00
MHDA- 1992/07/01 00:01
CRDT- 1992/07/01 00:00
PHST- 1992/07/01 00:00 [pubmed]
PHST- 1992/07/01 00:01 [medline]
PHST- 1992/07/01 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1992 Jul-Aug;12(4):1115-20.