PMID- 1324472
OWN - NLM
STAT- MEDLINE
DCOM- 19920923
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 20
IP  - 15
DP  - 1992 Aug 11
TI  - A shuttle vector which facilitates the expression of transfected genes in 
      Trypanosoma cruzi and Leishmania.
PG  - 3963-9
AB  - A Trypanosoma cruzi expression vector has been constructed using sequences 
      derived from the flanking regions of the glyceraldehyde 3-phosphate dehydrogenase 
      (gGAPDH) genes. The neomycin phosphotransferase (neor) gene was incorporated as a 
      selectable marker. Using electroporation we have introduced this vector into both 
      T. cruzi and Leishmania cells and conferred G418 resistance. Transformation is 
      mediated by large extrachromosomal circular elements composed of head-to-tail 
      tandem repeats of the vector. The transformed phenotype is stable for at least 6 
      months in the absence of G418 and can be maintained during passage through the T. 
      cruzi life-cycle. Foreign genes inserted into an expression site within the 
      vector (pTEX) can be expressed at high levels in transformed cells. To our 
      knowledge this paper describes the first trypanosome shuttle vector and the first 
      vector which functions in both trypanosomes and Leishmania.
FAU - Kelly, J M
AU  - Kelly JM
AD  - Department of Medical Parasitology, London School of Hygiene and Tropical 
      Medicine, UK.
FAU - Ward, H M
AU  - Ward HM
FAU - Miles, M A
AU  - Miles MA
FAU - Kendall, G
AU  - Kendall G
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 2.7.- (Phosphotransferases)
RN  - EC 2.7.1.95 (Kanamycin Kinase)
SB  - IM
GS  - gGAPDH
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Cloning, Molecular
MH  - Gene Expression/genetics
MH  - Genetic Vectors/*genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
MH  - Kanamycin Kinase
MH  - Leishmania donovani/*genetics
MH  - Leishmania mexicana/*genetics
MH  - Molecular Sequence Data
MH  - Phosphotransferases/genetics
MH  - Plasmids/*genetics
MH  - Recombinant Fusion Proteins/genetics
MH  - Transfection/genetics
MH  - Trypanosoma cruzi/*genetics
PMC - PMC334073
EDAT- 1992/08/11 00:00
MHDA- 1992/08/11 00:01
CRDT- 1992/08/11 00:00
PHST- 1992/08/11 00:00 [pubmed]
PHST- 1992/08/11 00:01 [medline]
PHST- 1992/08/11 00:00 [entrez]
AID - 10.1093/nar/20.15.3963 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1992 Aug 11;20(15):3963-9. doi: 10.1093/nar/20.15.3963.