PMID- 1326021
OWN - NLM
STAT- MEDLINE
DCOM- 19921014
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 52
IP  - 3
DP  - 1992 Sep
TI  - Inhibition site of dexamethasone on extravasation of polymorphonuclear leukocytes 
      in the hamster cheek pouch microcirculation.
PG  - 337-42
AB  - A video system was used to investigate the inhibitory effect(s) of the 
      glucocorticoid dexamethasone (DEX) on polymorphonuclear leukocyte (PMN) 
      extravasation in the microcirculation of hamster cheek pouch. DEX was given 
      intraperitoneally at 0.1 or 0.3 mg/kg body weight 2 h before induction of 
      extravasation by topical application of leukotriene B4 (LTB4) or 
      formyl-methionyl-leucyl-phenylalanine (fMLP) on the microvasculature. The number, 
      time course, and behavior of PMNs were examined in the following five steps of 
      extravasation: (1) rolling on the venular endothelium, (2) adhesion on the 
      endothelium, (3) passage between the endothelial cells, (4) staying in the 
      venular wall, and (5) migration from the venular wall into the interstitial 
      space. In either the presence or absence of DEX, topical application of LTB4 (15 
      pmol/50 microliters) or fMLP (10 nmol/50 microliters) caused an increase in the 
      number of PMNs that adhered to the venules. Thus, DEX did not inhibit the 
      adhesion of PMNs on the endothelial cells. The adhered PMNs induced by these 
      chemoattractants became gradually smaller, finally disappearing in the vascular 
      lumen, as observed on the monitor screen. The whole process took about 10 min. 
      This passage of PMNs between the endothelial cells was also not inhibited by DEX, 
      as over 90% of the adhered PMNs passed through the endothelial cells and advanced 
      to the next step of extravasation in the presence or absence of DEX. When these 
      chemoattractants were applied to DEX-untreated animals, the PMNs that passed 
      through the endothelial cells stayed for about 30 min in the venular wall. 
      Thereafter, PMNs migrated into the interstitial space. The numbers of PMNs in the 
      interstitial space were counted at 30, 60, and 90 min after the application of 
      chemoattractants. In the DEX-untreated animals, PMNs in the interstitial space 
      started to appear by 30 min, and thereafter the number further increased. 
      However, in the DEX-treated animals, the number of PMNs at 60 and 90 min was 
      significantly suppressed. Since DEX may inhibit the synthesis and/or release of a 
      proteinase(s) in the PMN granules, which would normally degrade the basement 
      membrane, this inhibition may be due to the inability of PMNs to penetrate the 
      basement membrane.
FAU - Oda, T
AU  - Oda T
AD  - Department of Pharmacology, Kitasato University School of Medicine, Sagamihara, 
      Japan.
FAU - Katori, M
AU  - Katori M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects/physiology
MH  - Cell Movement/drug effects/physiology
MH  - Cheek/blood supply
MH  - Cricetinae
MH  - Dexamethasone/administration & dosage/*pharmacology
MH  - Endothelium, Vascular/drug effects/physiology
MH  - Inflammation/physiopathology
MH  - Leukotriene B4/pharmacology
MH  - Microcirculation/drug effects
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*physiology
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
AID - 10.1002/jlb.52.3.337 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1992 Sep;52(3):337-42. doi: 10.1002/jlb.52.3.337.