PMID- 1326565
OWN - NLM
STAT- MEDLINE
DCOM- 19921019
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 119
IP  - 1
DP  - 1992 Oct
TI  - Inhibition of gap junction and adherens junction assembly by connexin and A-CAM 
      antibodies.
PG  - 179-89
AB  - We examined the roles of the extracellular domains of a gap junction protein and 
      a cell adhesion molecule in gap junction and adherens junction formation by 
      altering cell interactions with antibody Fab fragments. Using immunoblotting and 
      immunocytochemistry we demonstrated that Novikoff cells contained the gap 
      junction protein, connexin43 (Cx43), and the cell adhesion molecule, A-CAM 
      (N-cadherin). Cells were dissociated in EDTA, allowed to recover, and 
      reaggregated for 60 min in media containing Fab fragments prepared from a number 
      of antibodies. We observed no cell-cell dye transfer 4 min after microinjection 
      in 90% of the cell pairs treated with Fab fragments of antibodies for the first 
      or second extracellular domain of Cx43, the second extracellular domain of 
      connexin32 (Cx32) or A-CAM. Cell-cell dye transfer was detected within 30 s in 
      cell pairs treated with control Fab fragments (pre-immune serum, antibodies to 
      the rat major histocompatibility complex or the amino or carboxyl termii of 
      Cx43). We observed no gap junctions by freeze-fracture EM and no adherens 
      junctions by thin section EM between cells treated with the Fab fragments that 
      blocked cell-cell dye transfer. Gap junctions were found on approximately 50% of 
      the cells in control samples using freeze-fracture EM. We demonstrated with 
      reaggregated Novikoff cells that: (a) functional interactions of the 
      extracellular domains of the connexins were necessary for the formation of gap 
      junction channels; (b) cell interactions mediated by A-CAM were required for gap 
      junction assembly; and (c) Fab fragments of antibodies for A-CAM or connexin 
      extracellular domains blocked adherens junction formation.
FAU - Meyer, R A
AU  - Meyer RA
AD  - Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108.
FAU - Laird, D W
AU  - Laird DW
FAU - Revel, J P
AU  - Revel JP
FAU - Johnson, R G
AU  - Johnson RG
LA  - eng
GR  - CA-28548/CA/NCI NIH HHS/United States
GR  - GM46277/GM/NIGMS NIH HHS/United States
GR  - HL37109/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Antigens, CD)
RN  - 0 (CDH2 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Connexins)
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, CD
MH  - Cadherins
MH  - *Cell Adhesion
MH  - Cell Adhesion Molecules/immunology/*physiology
MH  - Connexins
MH  - Fluorescent Antibody Technique
MH  - Freeze Fracturing
MH  - Immunoglobulin Fab Fragments/*immunology
MH  - Intercellular Junctions/metabolism/*ultrastructure
MH  - Membrane Proteins/immunology/*physiology
MH  - Microscopy, Immunoelectron
MH  - Rats
MH  - Tumor Cells, Cultured
PMC - PMC2289623
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
PMCR- 1993/04/01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
PHST- 1993/04/01 00:00 [pmc-release]
AID - 92407032 [pii]
AID - 10.1083/jcb.119.1.179 [doi]
PST - ppublish
SO  - J Cell Biol. 1992 Oct;119(1):179-89. doi: 10.1083/jcb.119.1.179.