PMID- 1326649
OWN - NLM
STAT- MEDLINE
DCOM- 19921019
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 66
IP  - 10
DP  - 1992 Oct
TI  - Mapping of homologous, amino-terminal neutralizing regions of human T-cell 
      lymphotropic virus type I and II gp46 envelope glycoproteins.
PG  - 5879-89
AB  - Twelve synthetic peptides containing hydrophilic amino acid sequences of human 
      T-cell lymphotropic virus type I (HTLV-I) envelope glycoprotein were coupled to 
      tetanus toxoid and used to raise epitope-specific antisera in goats and rabbits. 
      Low neutralizing antibody titers (1:10 to 1:20) raised in rabbits to peptides 
      SP-2 (envelope amino acids [aa] 86 to 107), SP-3 (aa 176 to 189), and SP-4A (aa 
      190 to 209) as well as to combined peptide SP-3/4A (aa 176 to 209) were detected 
      in the vesicular stomatitis virus-HTLV-I pseudotype assay. Higher-titered 
      neutralizing antibody responses to HTLV-I (1:10 to 1:640) were detected with 
      pseudotype and syncytium inhibition assays in four goats immunized with a 
      combined inoculum containing peptides SP-2, SP-3, and SP-4A linked to tetanus 
      toxoid. These neutralizing anti-HTLV-I antibodies were type specific in that they 
      did not inhibit HTLV-II syncytium formation. Neutralizing antibodies in sera from 
      three goats could be absorbed with peptide SP-2 (aa 86 to 107) as well as 
      truncated peptides containing envelope aa 90 to 98, but not with equimolar 
      amounts of peptides lacking envelope aa 90 to 98. To map critical amino acids 
      that contributed to HTLV-I neutralization within aa 88 to 98, peptides in which 
      each amino acid was sequentially replaced by alanine were synthesized. The 
      resulting 11 synthetic peptides with single alanine substitutions were then used 
      to absorb three neutralizing goat antipeptide antisera. Both asparagines at 
      positions 93 and 95 were required for adsorption of neutralizing anti-HTLV-I 
      antibodies from all three sera. Peptide DP-90, containing the homologous region 
      of HTLV-II envelope glycoprotein (aa 82 to 97), elicited antipeptide neutralizing 
      antibodies to HTLV-II in goats that were type specific. In further adsorption 
      experiments, it was determined that amino acid differences between homologous 
      HTLV-I and HTLV-II envelope sequences at HTLV-I aa 95 (N to Q) and 97 (G to L) 
      determined the type specificity of these neutralizing sites. Thus, the 
      amino-terminal regions of HTLV-I and -II gp46 contain homologous, linear, 
      neutralizing determinants that are type specific.
FAU - Palker, T J
AU  - Palker TJ
AD  - Department of Medicine, Duke University Medical Center, Durham, North Carolina.
FAU - Riggs, E R
AU  - Riggs ER
FAU - Spragion, D E
AU  - Spragion DE
FAU - Muir, A J
AU  - Muir AJ
FAU - Scearce, R M
AU  - Scearce RM
FAU - Randall, R R
AU  - Randall RR
FAU - McAdams, M W
AU  - McAdams MW
FAU - McKnight, A
AU  - McKnight A
FAU - Clapham, P R
AU  - Clapham PR
FAU - Weiss, R A
AU  - Weiss RA
AU  - et al.
LA  - eng
GR  - 1P30-AI28662/AI/NIAID NIH HHS/United States
GR  - CA-440660/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Gene Products, env)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (HTLV-II Antibodies)
RN  - 0 (Immune Sera)
RN  - 0 (Peptides)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (env Gene Products, Human Immunodeficiency Virus)
RN  - 0 (gp46 protein, Human T-cell leukemia virus type I)
RN  - 0 (gp46 protein, Human immunodeficiency virus 2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western
MH  - Gene Products, env/genetics/*immunology
MH  - Goats
MH  - HTLV-I Antibodies/immunology
MH  - HTLV-II Antibodies/immunology
MH  - Immune Sera
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Neutralization Tests
MH  - Peptides/*genetics
MH  - Rabbits
MH  - Radioimmunoassay
MH  - Retroviridae Proteins, Oncogenic/genetics/*immunology
MH  - Vesicular stomatitis Indiana virus/metabolism
MH  - env Gene Products, Human Immunodeficiency Virus
PMC - PMC241464
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
PMCR- 1992/10/01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
PHST- 1992/10/01 00:00 [pmc-release]
AID - 10.1128/JVI.66.10.5879-5889.1992 [doi]
PST - ppublish
SO  - J Virol. 1992 Oct;66(10):5879-89. doi: 10.1128/JVI.66.10.5879-5889.1992.