PMID- 1327070
OWN - NLM
STAT- MEDLINE
DCOM- 19921113
LR  - 20190827
IS  - 0960-0760 (Print)
IS  - 0960-0760 (Linking)
VI  - 43
IP  - 5
DP  - 1992 Oct
TI  - Interplay of steroid hormone receptors and transcription factors on the mouse 
      mammary tumor virus promoter.
PG  - 365-78
AB  - The mouse mammary tumor virus (MMTV) promoter, that responds to glucocorticoids 
      and progestins, contains a complex hormone response element (HRE) in the long 
      terminal repeat (LTR) region covered by a phased nucleosome. Hormone treatment 
      leads to alterations in chromatin structure that make the HRE region more 
      accessible to digestion by DNase I and permit binding of transcription factors, 
      including nuclear factor I (NFI), immediately downstream of the HRE. NFI acts as 
      a basal transcription factor on the MMTV promoter in vitro but competes with the 
      hormone receptors in terms of binding to free DNA. In uninduced chromatin, the 
      precise positioning of the DNA double helix on the surface of the histone octamer 
      precludes binding of NFI to its cognate sequence while still allowing recognition 
      of the HRE by the hormone receptors. We postulate that receptor binding to the 
      nucleosomally organized MMTV promoter disrupts the chromatin structure enabling 
      NFI binding and subsequent formation of a stable transcription complex. Whether 
      the receptor remains bound to DNA during induction or is displaced by NFI is not 
      conclusively known, but our evidence supports a "hit and run" mechanism. NFI is 
      not the only factor involved in hormonally induced transcription of the MMTV 
      promoter. Two degenerated octamer motifs located immediately upstream of the TATA 
      box are recognized by the ubiquitous transcription factor OTF-1 (Oct-1, NFIII), 
      and are also important. In vitro, mutations in these motifs do not influence 
      basal transcription, but completely abolish the stimulatory effect of purified 
      progesterone receptor. Progesterone receptor bound to the HRE facilitates binding 
      of OTF-1 to the two octamer motifs. Thus, OTF-1 is a natural mediator of 
      progesterone induction of the MMTV promoter and acts through cooperation with the 
      hormone receptor for binding to DNA.
FAU - Truss, M
AU  - Truss M
AD  - Institut fur Molekularbiologie und Tumorforschung, Marburg, Germany.
FAU - Chalepakis, G
AU  - Chalepakis G
FAU - Beato, M
AU  - Beato M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Transcription Factors)
RN  - 0 (Y-Box-Binding Protein 1)
RN  - 0 (YBX1 protein, human)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - DNA/analysis
MH  - DNA-Binding Proteins/physiology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - NFI Transcription Factors
MH  - Nuclear Proteins
MH  - Rabbits
MH  - Rats
MH  - Receptors, Glucocorticoid/*physiology
MH  - Receptors, Progesterone/*physiology
MH  - Transcription Factors/*physiology
MH  - Y-Box-Binding Protein 1
RF  - 104
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
AID - 0960-0760(92)90071-P [pii]
AID - 10.1016/0960-0760(92)90071-p [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 1992 Oct;43(5):365-78. doi: 
      10.1016/0960-0760(92)90071-p.