PMID- 1332051
OWN - NLM
STAT- MEDLINE
DCOM- 19921211
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 89
IP  - 21
DP  - 1992 Nov 1
TI  - Human herpesvirus 7 is a T-lymphotropic virus and is related to, but 
      significantly different from, human herpesvirus 6 and human cytomegalovirus.
PG  - 10552-6
AB  - An independent strain (JI) of human herpesvirus 7 (HHV-7) was isolated from a 
      patient with chronic fatigue syndrome (CFS). No significant association could be 
      established by seroepidemiology between HHV-7 and CFS. HHV-7 is a T-lymphotropic 
      virus, infecting CD4+ and CD8+ primary lymphocytes. HHV-7 can also infect SUP-T1, 
      an immature T-cell line, with variable success. Southern blot analysis with DNA 
      probes scanning 58.8% of the human herpesvirus 6 (HHV-6) genome and hybridizing 
      to all HHV-6 strains tested so far revealed homology to HHV-7 with only 37.4% of 
      the total probe length. HHV-7 contains the GGGTTA repetitive sequence, as do 
      HHV-6 and Marek's disease chicken herpesvirus. DNA sequencing of a 186-base-pair 
      fragment of HHV-7(JI) revealed an identity with HHV-6 and human cytomegalovirus 
      of 57.5% and 36%, respectively. Oligonucleotide primers derived from this 
      sequence (HV7/HV8, HV10/HV11) amplified HHV-7 DNA only and did not amplify DNA 
      from other human herpesviruses, including 12 different HHV-6 strains. Southern 
      blot analysis with the p43L3 probe containing the 186-base-pair HHV-7 DNA 
      fragment hybridized to HHV-7 DNA only. The molecular divergence between human 
      cytomegalovirus, on the one hand, and HHV-6 and HHV-7, on the other, is greater 
      than between HHV-6 and HHV-7, which, in turn, is greater than the difference 
      between HHV-6 strains. This study supports the classification of HHV-7 as an 
      additional member of the human beta-herpesviruses.
FAU - Berneman, Z N
AU  - Berneman ZN
AD  - Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes 
      of Health, Bethesda, MD 20892.
FAU - Ablashi, D V
AU  - Ablashi DV
FAU - Li, G
AU  - Li G
FAU - Eger-Fletcher, M
AU  - Eger-Fletcher M
FAU - Reitz, M S Jr
AU  - Reitz MS Jr
FAU - Hung, C L
AU  - Hung CL
FAU - Brus, I
AU  - Brus I
FAU - Komaroff, A L
AU  - Komaroff AL
FAU - Gallo, R C
AU  - Gallo RC
LA  - eng
SI  - GENBANK/L03525
SI  - GENBANK/L03526
GR  - R01AI26788/AI/NIAID NIH HHS/United States
GR  - R01AI27314/AI/NIAID NIH HHS/United States
GR  - U01AI32246/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CD4 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Viral)
RN  - 0 (Oligodeoxyribonucleotides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Southern
MH  - CD4 Antigens/immunology
MH  - CD8 Antigens/immunology
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cytomegalovirus/*classification/*genetics
MH  - DNA Probes
MH  - DNA, Viral/genetics
MH  - Herpesvirus 6, Human/*classification/*genetics
MH  - Herpesvirus 7, Human/*classification/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides
MH  - Polymerase Chain Reaction/methods
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - T-Lymphocytes/microbiology
PMC - PMC50377
EDAT- 1992/11/11 19:15
MHDA- 2001/03/28 10:01
PMCR- 1993/05/01
CRDT- 1992/11/11 19:15
PHST- 1992/11/11 19:15 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1992/11/11 19:15 [entrez]
PHST- 1993/05/01 00:00 [pmc-release]
AID - 10.1073/pnas.89.21.10552 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10552-6. doi: 
      10.1073/pnas.89.21.10552.