PMID- 1333126
OWN - NLM
STAT- MEDLINE
DCOM- 19921230
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 191
IP  - 2
DP  - 1992 Dec
TI  - Molecular cloning and nucleotide sequence of a pestivirus genome, noncytopathic 
      bovine viral diarrhea virus strain SD-1.
PG  - 867-9
AB  - Genomic RNA of noncytopathic (NCP) bovine viral diarrhea virus (BVDV) strain SD-1 
      was extracted directly from serum obtained from a persistently infected animal. 
      cDNA was synthesized and amplified by polymerase chain reaction (PCR) before 
      cloning. The complete genomic nucleotide sequence was determined by sequencing at 
      least two different clones from independent PCR reactions. The 5' and 3' end 
      sequences of the SD-1 genome was determined from 5'-3' ligation clones. The 
      complete genome sequence was comprised of 12,308 nucleotides containing one large 
      open reading frame which encodes an amino acid sequence of 3898 residues with a 
      calculated molecular weight of 438 kDa. In contrast to cytopathic (CP) BVDV 
      strain NADL, which contains a cellular RNA insert of 270 nucleotides and CP BVDV 
      strain Osloss, which has an inserted ubiquitin RNA sequence of 228 nucleotides, 
      the NCP strain SD-1 had no insertion along the genome. Sequence comparison with 
      other pestiviruses revealed that the overall nucleotide sequence homologies of 
      SD-1 are 88.6% with NADL, 78.3% with Osloss, 67.1% with HoCV Alfort, and 67.2% 
      with HoCV Brescia. The overall deduced amino acid sequence homologies of SD-1 are 
      92.7% with NADL, 86.2% with Osloss, 72.5% with HoCV Alfort, and 71.2% with HoCV 
      Brescia. The most conserved nucleotide and amino acid sequences are located in 
      the 5' untranslated region (5'UTR) and nonstructural protein p80 region, 
      respectively. The viral glycoproteins, particularly gp53, and nonstructural 
      proteins p54 and p58 have the lowest homology comparing both nucleotide and amino 
      acid sequences between SD-1 and other pestiviruses. Extensive analyses of amino 
      acid sequences for the viral structural proteins and nonstructural protein p54 
      regions from five pestiviruses led to the identification of four conserved 
      domains (designated as C1, C2, C3, C4) and three highly variable domains 
      (designated as V1, V2, V3) within this region. The C1, C2, and C3 domains are 
      located in the capsid protein p14, glycoprotein gp48, and gp25, respectively. The 
      C4 domain is located in the junction between gp53 and p54. Interestingly, out of 
      three variable domains, two (V1, V2) are located in the same glycoprotein gp53. 
      The third variable domain is located in the nonstructural protein p54.
FAU - Deng, R
AU  - Deng R
AD  - Ohio Agricultural Research And Development Center, Food Animal Health Research 
      Program, Wooster 44691.
FAU - Brock, K V
AU  - Brock KV
LA  - eng
SI  - GENBANK/M96751
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cloning, Molecular
MH  - Cytopathogenic Effect, Viral
MH  - Genetic Variation
MH  - Genome, Viral
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Pestivirus/*genetics
MH  - Polymerase Chain Reaction
MH  - Protein Precursors/genetics
MH  - Protein Structure, Secondary
MH  - RNA, Viral/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Viral Proteins/genetics
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1016/0042-6822(92)90262-n [doi]
PST - ppublish
SO  - Virology. 1992 Dec;191(2):867-9. doi: 10.1016/0042-6822(92)90262-n.