PMID- 1333176
OWN - NLM
STAT- MEDLINE
DCOM- 19921229
LR  - 20061115
IS  - 0364-5134 (Print)
IS  - 0364-5134 (Linking)
VI  - 32
IP  - 5
DP  - 1992 Nov
TI  - No evidence for spumavirus or oncovirus infection in relapsing-remitting multiple 
      sclerosis.
PG  - 711-4
AB  - Polymerase chain reaction analysis was used to investigate the possible role of 
      human spumaretrovirus and oncoretroviruses (human T-cell lymphotropic virus types 
      I [HTLV-I] and II [HTLV-II]) in multiple sclerosis. Eleven patients with 
      relapsing-remitting multiple sclerosis in exacerbation and 11 normal blood donors 
      were included in the study. Cerebrospinal fluid cells, peripheral blood 
      mononuclear cells, and plasma were cocultured with allogeneic mononuclear cells 
      for 6 weeks. Cultured cells were subjected to polymerase chain reaction analysis 
      with primers selected for the pol and gag (human spumaretrovirus), pol and env 
      (HTLV-I), and pol (HTLV-II) genes. Polymerase chain reaction was negative in all 
      patient and blood donor control samples, whereas positive controls were 
      consistently reactive with high sensitivity. No culture exhibited cytopathic 
      effects and supernatants were negative for reverse transcriptase activity. Thus, 
      our results do not support a role for these retroviruses in the pathogenesis of 
      multiple sclerosis.
FAU - Svenningsson, A
AU  - Svenningsson A
AD  - Department of Clinical Chemistry, Sahlgren's Hospital, Gothenburg, Sweden.
FAU - Lycke, J
AU  - Lycke J
FAU - Svennerholm, B
AU  - Svennerholm B
FAU - Gronowitz, S
AU  - Gronowitz S
FAU - Andersen, O
AU  - Andersen O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann Neurol
JT  - Annals of neurology
JID - 7707449
RN  - 0 (DNA, Viral)
RN  - 0 (HTLV-I Antigens)
SB  - IM
GS  - env
GS  - gag
GS  - pol
MH  - Adult
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Cells, Cultured
MH  - DNA, Viral/analysis
MH  - Female
MH  - Gene Amplification
MH  - HTLV-I Antigens/analysis
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Multiple Sclerosis/cerebrospinal fluid/*microbiology
MH  - Polymerase Chain Reaction
MH  - Retroviridae/isolation & purification
MH  - Retroviridae Infections/cerebrospinal fluid/*microbiology
MH  - Sensitivity and Specificity
MH  - Spumavirus/isolation & purification
MH  - Tumor Virus Infections/cerebrospinal fluid/*microbiology
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 10.1002/ana.410320518 [doi]
PST - ppublish
SO  - Ann Neurol. 1992 Nov;32(5):711-4. doi: 10.1002/ana.410320518.