PMID- 1333251
OWN - NLM
STAT- MEDLINE
DCOM- 19930104
LR  - 20181130
IS  - 0934-9820 (Print)
IS  - 0934-9820 (Linking)
VI  - 5 Suppl
DP  - 1992
TI  - Functionally diverse purinergic P2Y-receptors mediate prostanoid synthesis in 
      cultured rat astrocytes: the role of ATP-induced phosphatidyl-inositol breakdown.
PG  - S19-22
AB  - Cultured rat astrocytes possess purinergic P2Y-receptors. Stimulation of these 
      receptors with ATP (10(-3) M) results in increased phosphatidylinositol 
      biphosphate (PIP2)-breakdown and prostanoid formation. We have investigated the 
      relevance of the PIP2-pathway in prostanoid synthesis. The intracellular 
      Ca(2+)-mobilizing agent thapsigargin (TG) (10(-6) M) and the diacylglycerol 
      (DAG)-mimetic tetradecaoylphorbol acetate (TPA) (10(-8)-10(-6) M) both stimulate 
      prostaglandin D2 production. ATP-induced prostanoid formation can be mimicked by 
      combined addition of TG and TPA, suggesting the importance of the second 
      messengers IP3 and DAG, generated during P2Y-receptor mediated PIP2-breakdown. 
      Inhibition of ATP-induced PIP2-hydrolysis by TPA (IC50 about 5 x 10(-8) M) or by 
      10(-4) M neomycine, however, does not affect astroglial prostanoid synthesis, 
      showing that P2Y-receptor mediated prostanoid formation may occur also in the 
      absence of PIP2-hydrolysis. These findings suggest that additional postreceptor 
      mechanisms exist in the signal transduction chain of ATP-induced astroglial 
      prostanoid synthesis. A possible involvement of phospholipase A2 and/or of 
      Ca(2+)-channels, directly coupled to P2Y-receptors is proposed.
FAU - Seregi, A
AU  - Seregi A
AD  - Department of Pharamcology, University of Freiburg, FRG.
FAU - Doll, S
AU  - Doll S
FAU - Schobert, A
AU  - Schobert A
FAU - Hertting, G
AU  - Hertting G
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Eicosanoids
JT  - Eicosanoids
JID - 8906009
RN  - 0 (Phosphatidylinositol 4,5-Diphosphate)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Prostaglandins)
RN  - 0 (Receptors, Purinergic)
RN  - 0 (Terpenes)
RN  - 67526-95-8 (Thapsigargin)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - I16QD7X297 (Neomycin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - RXY07S6CZ2 (Prostaglandin D2)
SB  - IM
MH  - Adenosine Triphosphate/*pharmacology
MH  - Animals
MH  - Astrocytes/drug effects/*metabolism
MH  - Cells, Cultured
MH  - Neomycin/pharmacology
MH  - Phosphatidylinositol 4,5-Diphosphate
MH  - Phosphatidylinositols/*metabolism
MH  - Prostaglandin D2/biosynthesis
MH  - Prostaglandins/*biosynthesis
MH  - Rats
MH  - Receptors, Purinergic/drug effects/*physiology
MH  - Terpenes/pharmacology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Thapsigargin
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
PST - ppublish
SO  - Eicosanoids. 1992;5 Suppl:S19-22.