PMID- 1333596
OWN - NLM
STAT- MEDLINE
DCOM- 19921231
LR  - 20190902
IS  - 0901-9928 (Print)
IS  - 0901-9928 (Linking)
VI  - 71
IP  - 4
DP  - 1992 Oct
TI  - Exogenous GTP increases cyclic GMP and inhibits thrombin-induced aggregation of 
      washed human platelets: comparison with ATP, adenosine and guanosine.
PG  - 289-93
AB  - The effects of exogenous guanosine 5'-triphosphate (GTP), guanosine, adenosine 
      5'-triphosphate (ATP) and adenosine on platelet aggregation, serotonin secretion 
      and cyclic nucleotide accumulation were studied using thrombin-stimulated washed 
      human platelets. GTP (10 microM-1 mM) dose-dependently inhibited thrombin-induced 
      aggregation and serotonin secretion. The inhibition of aggregation was 
      accompanied by an increase in platelet cyclic GMP. GTP did not affect cyclic AMP 
      concentration. Adenosine (1 microM-1 mM) dose-dependently inhibited 
      thrombin-induced aggregation and serotonin secretion, and increased cyclic AMP. 
      ATP at high concentrations (100 microM-1 mM) inhibited aggregation and serotonin 
      secretion, and 1 mM ATP increased cyclic AMP. Guanosine was relatively 
      ineffective in preventing aggregation and serotonin secretion and did not affect 
      cyclic GMP. The rank order of inhibition of thrombin-induced aggregation of 
      washed human platelets was adenosine > GTP > ATP > guanosine. In conclusion, 
      exogenous GTP inhibits thrombin-induced aggregation and serotonin secretion of 
      washed human platelets by increasing cyclic GMP. The results raise the 
      possibility of a cell membrane site of action for GTP in platelets which mediates 
      the activation of soluble guanylate cyclase suggesting that GTP may have a local 
      antithrombotic effect also in vivo.
FAU - Vuorinen, P
AU  - Vuorinen P
AD  - Department of Biomedical Sciences, University of Tampere, Finland.
FAU - Laustiola, K E
AU  - Laustiola KE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Pharmacol Toxicol
JT  - Pharmacology & toxicology
JID - 8702180
RN  - 0 (Platelet Aggregation Inhibitors)
RN  - 12133JR80S (Guanosine)
RN  - 333DO1RDJY (Serotonin)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.4.21.5 (Thrombin)
RN  - H2D2X058MU (Cyclic GMP)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine/pharmacology
MH  - Adenosine Triphosphate/*pharmacology
MH  - Blood Platelets/*drug effects
MH  - Cyclic GMP/*metabolism
MH  - Guanosine/pharmacology
MH  - Guanosine Triphosphate/*pharmacology
MH  - Humans
MH  - Platelet Aggregation/*drug effects
MH  - Platelet Aggregation Inhibitors/*pharmacology
MH  - Serotonin/metabolism
MH  - Thrombin/*antagonists & inhibitors/pharmacology
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
AID - 10.1111/j.1600-0773.1992.tb00985.x [doi]
PST - ppublish
SO  - Pharmacol Toxicol. 1992 Oct;71(4):289-93. doi: 
      10.1111/j.1600-0773.1992.tb00985.x.