PMID- 1333982
OWN - NLM
STAT- MEDLINE
DCOM- 19930112
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 203
IP  - 2
DP  - 1992 Dec
TI  - Selective localization of receptors for urokinase amino-terminal fragment at 
      substratum contact sites of an in vitro-established line of human epidermal 
      cells.
PG  - 427-34
AB  - We have shown the presence of surface receptors for the amino-terminal fragment 
      (ATF) of human urokinase-type plasminogen activator (u-PA) on an in 
      vitro-established cell line of human epidermal origin by both radio-binding 
      assays with human 125I-u-PA-ATF and transmission electron microscopy of a 
      gold-u-PA complex. On the basis of cross-linking experiments with 125I-u-PA-ATF 
      and subsequent autoradiography of the gels we have observed that such receptors 
      are not spontaneously released into the culture medium. The treatment with 
      phosphatidylinositol-specific phospholipase C induces the release of the 
      receptor, which behaves as a glycosyl phosphatidyl inositol(GPI)-anchored 
      protein. Phase-partitioning experiments on cell lysates have shown that the 
      receptor partitions into the detergent phase. By detaching cell monolayers with 
      the chelating agent EDTA we have prepared the cell-substratum contact sites of 
      these cells, which represent only the 3.5% of the surface membrane of monolayered 
      cells. Such plasma membrane remnants are highly selected since they contain about 
      43% of total u-PA-ATF binding sites. Such binding sites show the same biochemical 
      and morphological characteristics of u-PA-ATF receptors observed in the 
      monolayered cells, thus indicating that u-PA is selectively concentrated at the 
      level of cell-substratum contacts. This is likely to enable directional 
      proteolysis for cell migration and invasion.
FAU - Del Rosso, M
AU  - Del Rosso M
AD  - Istituto di Patologia Generale, Universita di Firenze, Italy.
FAU - Pedersen, N
AU  - Pedersen N
FAU - Fibbi, G
AU  - Fibbi G
FAU - Pucci, M
AU  - Pucci M
FAU - Dini, G
AU  - Dini G
FAU - Anichini, E
AU  - Anichini E
FAU - Blasi, F
AU  - Blasi F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (PLAUR protein, human)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - 0 (Succinimides)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.1.4.11 (Phosphoinositide Phospholipase C)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase)
RN  - V9WYZ7QMDT (disuccinimidyl suberate)
SB  - IM
MH  - Binding Sites
MH  - *Cell Adhesion
MH  - Cell Line
MH  - Cell Membrane/chemistry/ultrastructure
MH  - Cross-Linking Reagents/pharmacology
MH  - Epidermis/*chemistry/ultrastructure
MH  - Humans
MH  - Microscopy, Electron
MH  - Peptide Fragments/*metabolism
MH  - Phosphatidylinositol Diacylglycerol-Lyase
MH  - Phosphoinositide Phospholipase C
MH  - Phosphoric Diester Hydrolases/pharmacology
MH  - Receptors, Cell Surface/*analysis
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Succinimides/pharmacology
MH  - Urokinase-Type Plasminogen Activator/*metabolism
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1016/0014-4827(92)90017-3 [doi]
PST - ppublish
SO  - Exp Cell Res. 1992 Dec;203(2):427-34. doi: 10.1016/0014-4827(92)90017-3.