PMID- 1335762
OWN - NLM
STAT- MEDLINE
DCOM- 19930211
LR  - 20071115
IS  - 1056-5477 (Print)
IS  - 1056-5477 (Linking)
VI  - 11
IP  - 6
DP  - 1992 Dec
TI  - Down-modulation of cell surface expression of p80 form of the tumor necrosis 
      factor receptor by human immunodeficiency virus-1 tat gene.
PG  - 317-25
AB  - Tumor necrosis factor-alpha (TNF-alpha) induces the expression of human 
      immunodeficiency virus type-1 (HIV-1) in vitro in chronically infected cells of T 
      and monocytic origin. The tat protein from the HIV-1 virus has been shown to be 
      essential for HIV replication and in the immunosuppression associated with the 
      virus infection. Previous studies in our laboratory have shown that HIV-1 tat 
      gene induces TNF-beta (lymphotoxin) in human B-lymphoblastoid cells (Sastry et 
      al., 1990, J. Biol. Chem. 265, 20091-20093). In an attempt to characterize 
      further the relationship between the host and HIV-1, we investigated the effect 
      of the functional HIV-1 tat gene on the expression of TNF receptors in a human B 
      lymphoblastoid cell line (Raji). We report here that Raji cells transfected with 
      HIV-1 tat gene express fewer cell surface TNF receptors than control cells. At 
      least a 5-fold decrease in the receptor number without any significant change in 
      receptor affinity was observed. The decrease in TNF receptors in tat-transfected 
      Raji cells (Raji-tat cells) was found not to be due to receptor occupancy by the 
      autocrine production of TNF-beta. The decrease in the cell surface expression of 
      TNF receptors in Raji-tat cells was also found to be not due to a decrease in the 
      gene expression of the receptor. The kinetics, amount of TNF binding and its 
      internalization were temperature dependent, and it was different in Raji-tat 
      cells than in the control cells.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Pocsik, E
AU  - Pocsik E
AD  - Department of Clinical Immunology and Biological Therapy, University of Texas M. 
      D. Anderson Cancer, Houston 77030.
FAU - Higuchi, M
AU  - Higuchi M
FAU - Aggarwal, B B
AU  - Aggarwal BB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lymphokine Cytokine Res
JT  - Lymphokine and cytokine research
JID - 9107882
RN  - 0 (Gene Products, tat)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
SB  - IM
GS  - tat
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Down-Regulation
MH  - Gene Expression
MH  - Gene Products, tat/genetics/immunology/metabolism
MH  - *Genes, tat
MH  - HIV-1/*genetics/immunology
MH  - Humans
MH  - Immune Tolerance
MH  - Molecular Weight
MH  - Receptors, Cell Surface/chemistry/*metabolism
MH  - Receptors, Tumor Necrosis Factor
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - tat Gene Products, Human Immunodeficiency Virus
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
PST - ppublish
SO  - Lymphokine Cytokine Res. 1992 Dec;11(6):317-25.