PMID- 1335785
OWN - NLM
STAT- MEDLINE
DCOM- 19930205
LR  - 20181130
IS  - 0956-960X (Print)
IS  - 0956-960X (Linking)
VI  - 3
IP  - 4
DP  - 1992 Oct
TI  - Protein kinase C regulates secretion of lymphotoxin (LT/TNF beta) and TNF alpha 
      by human EBV-transformed B cells.
PG  - 162-7
AB  - Epstein-Barr virus (EBV)-transformed cell lines constitutively secrete 
      lymphotoxin (LT/TNF beta) and not tumor necrosis factor-alpha (TNF alpha). To 
      analyze the cellular processes that regulate LT and TNF alpha secretion by 
      lymphoblastoid cell lines, we studied the role of two signal transduction 
      pathways leading to either protein kinase C (PK-C) or PK-A activation. We 
      demonstrate that PK-C activation, either after cross-linking of surface Ig or by 
      direct activation with phorbolester, leads to increased production of both LT and 
      TNF alpha, whereas no prominent role for PK-A was found. Interleukin (Il)-4 was 
      found to synergize with PK-C activation in raising levels of secreted LT and TNF 
      alpha. Increased levels of LT and TNF alpha did not correlate with augmented 
      levels of immunoglobulin secreted by the cell lines nor with improved 
      proliferation. These observations demonstrate that EBV B cells respond to B cell 
      activation signals leading to PK-C activation with increased production of both 
      LT and TNF alpha. It is, however, unlikely that these molecules serve as 
      autostimulatory factors for EBV B cells, but in contrast might play a role in 
      downregulation of biological functions in these cells.
FAU - Jochems, G J
AU  - Jochems GJ
AD  - Department of Clinical (Viro-)Immunology, Central Laboratory of the Netherlands 
      Red Cross Blood Transfusion Service, Amsterdam.
FAU - Klein, M R
AU  - Klein MR
FAU - Zeijlemaker, W P
AU  - Zeijlemaker WP
FAU - van Lier, R A
AU  - van Lier RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Antibodies Hybridomas
JT  - Human antibodies and hybridomas
JID - 9014461
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Phosphatidylinositol 4,5-Diphosphate)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - B-Lymphocytes/immunology/*physiology
MH  - Cell Line, Transformed
MH  - Herpesvirus 4, Human
MH  - Humans
MH  - Lymphocyte Activation
MH  - Lymphotoxin-alpha/*metabolism
MH  - Phosphatidylinositol 4,5-Diphosphate
MH  - Phosphatidylinositols/metabolism
MH  - Protein Kinase C/*physiology
MH  - Protein Kinases/physiology
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
PST - ppublish
SO  - Hum Antibodies Hybridomas. 1992 Oct;3(4):162-7.