PMID- 1336558
OWN - NLM
STAT- MEDLINE
DCOM- 19930212
LR  - 20191210
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 33
IP  - 4
DP  - 1992 Dec
TI  - Differential regulation of the nerve growth factor and brain-derived neurotrophic 
      factor genes in L929 mouse fibroblasts.
PG  - 519-26
AB  - Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are 
      structurally related survival and differentiation factors for distinct sets of 
      peripheral and central neurons. The regulation of NGF gene expression has been 
      extensively studied in L929 mouse fibroblasts. L929 cells also express the BDNF 
      gene. Northern blot hybridization analysis revealed 4 discrete BDNF mRNA species 
      in L929 cells and rat hippocampus after induction of seizures with kainic acid. 
      Serum as well as 12-O-tetradecanoyl phorbol-13-acetate (TPA) stimulated NGF and 
      all 4 BDNF mRNAs in L929 cells. Treatment with both agents induced NGF mRNA to a 
      much larger extent than the BDNF mRNAs. The induction of the BDNF mRNAs was 
      rapid, with nearly maximal levels by 1 hr. In contrast, NGF mRNA induction 
      occurred later and peaked at 4-6 hr. Both NGF and BDNF mRNA induction were 
      inhibited by actinomycin D. Cycloheximide, on the other hand, inhibited only NGF 
      but not BDNF mRNA induction. Corticosterone rapidly decreased NGF mRNA but not 
      the BDNF mRNAs, and had no effect on seizure-induced NGF or BDNF mRNAs. Forskolin 
      did not stimulate NGF or BDNF mRNAs. In contrast to NGF mRNA, forskolin did not 
      interfere with the serum induction of BDNF mRNAs. These results demonstrate that 
      2 genes which encode closely related neurotrophic factors are differentially 
      regulated in L929 cells. The molecular mechanisms which bring about this 
      differential regulation remain to be elucidated.
FAU - D'Mello, S R
AU  - D'Mello SR
AD  - Evans Department of Clinical Research, University Hospital, Boston, Massachusetts 
      02118.
FAU - Jiang, C
AU  - Jiang C
FAU - Lamberti, C
AU  - Lamberti C
FAU - Martin, S C
AU  - Martin SC
FAU - Heinrich, G
AU  - Heinrich G
LA  - eng
GR  - NS22422/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 1F7A44V6OU (Colforsin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SIV03811UC (Kainic Acid)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Brain-Derived Neurotrophic Factor
MH  - Colforsin/pharmacology
MH  - Corticosterone/pharmacology
MH  - Cyclic AMP/metabolism
MH  - Fibroblasts/drug effects/physiology
MH  - *Gene Expression Regulation/drug effects
MH  - Hippocampus/drug effects/*metabolism
MH  - Kainic Acid
MH  - L Cells
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/*genetics
MH  - Nerve Tissue Proteins/*genetics
MH  - Oligodeoxyribonucleotides
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seizures/chemically induced/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1002/jnr.490330404 [doi]
PST - ppublish
SO  - J Neurosci Res. 1992 Dec;33(4):519-26. doi: 10.1002/jnr.490330404.