PMID- 1337259
OWN - NLM
STAT- MEDLINE
DCOM- 19930309
LR  - 20131121
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 8
IP  - 12
DP  - 1992 Dec
TI  - Infection by human herpesvirus 6 (HHV-6) of human lymphoid T cells occurs through 
      an endocytic pathway.
PG  - 2031-7
AB  - We have analyzed by immunoelectron microscopy the early events of binding and 
      internalization of human herpesvirus 6 (HHV-6, strain GS) on a susceptible 
      T-lymphoblastoid cell line, HSB-2. The virions bound to the cell surface at 4 
      degrees C were tightly associated with the plasma membrane. Gold immunolabeling 
      of the viral envelope proteins was strong and specific. Warming at 37 degrees C 
      for different times showed viral internalization through smooth surfaced pits and 
      vesicles. Fusion events of the virions with the cell plasma membrane were never 
      observed. Gold immunolabeling performed in parallel experiments before or after 
      viral internalization showed: (1) absence of viral envelope proteins on the cell 
      plasma membranes at all times of internalization, again excluding fusion events; 
      (2) entry of the virions with their envelopes. Treatment of the cells with 
      chloroquine, a drug known to affect the endocytic pathway, led to an almost 
      complete inhibition of viral infectivity, suggesting that the endocytosed virions 
      are responsible for a successful infection. Comparable results were obtained 
      using a second strain of HHV-6 (BA92), with biologic and molecular 
      characteristics similar to the prototype strain Z29. The chloroquine inhibition 
      was effective on two different T cell lines (HSB-2 and J-Jhan), as well as on 
      phytohemagglutinin-stimulated peripheral blood mononuclear cells.
FAU - Cirone, M
AU  - Cirone M
AD  - Dipartimento di Medicina Sperimentale, Universita degli Studi La Sapienza, Rome, 
      Italy.
FAU - Zompetta, C
AU  - Zompetta C
FAU - Angeloni, A
AU  - Angeloni A
FAU - Ablashi, D V
AU  - Ablashi DV
FAU - Salahuddin, S Z
AU  - Salahuddin SZ
FAU - Pavan, A
AU  - Pavan A
FAU - Torrisi, M R
AU  - Torrisi MR
FAU - Frati, L
AU  - Frati L
FAU - Faggioni, A
AU  - Faggioni A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 886U3H6UFF (Chloroquine)
SB  - IM
MH  - Cell Line
MH  - Chloroquine/pharmacology
MH  - Endocytosis/drug effects
MH  - Herpesviridae Infections/*etiology/microbiology/pathology
MH  - *Herpesvirus 6, Human/drug effects/ultrastructure
MH  - Humans
MH  - Microscopy, Immunoelectron
MH  - T-Lymphocytes/drug effects/*microbiology/ultrastructure
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1089/aid.1992.8.2031 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 1992 Dec;8(12):2031-7. doi: 10.1089/aid.1992.8.2031.