PMID- 1337928
OWN - NLM
STAT- MEDLINE
DCOM- 19930401
LR  - 20190824
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 16
IP  - 3-4
DP  - 1992 Dec
TI  - Brain aromatase cytochrome P-450 messenger RNA levels and enzyme activity during 
      prenatal and perinatal development in the rat.
PG  - 187-92
AB  - Aromatase cytochrome P-450 (P-450AROM) enzyme activity catalyzes the conversion 
      of androgens to estrogens in specific brain areas. During development local 
      estrogen formation is thought to influence the sexual differentiation of neural 
      structures (i.e. increase neurite growth and establish neural circuitry) and 
      modulate reproductive functions. This study was undertaken to investigate the 
      ontogeny of the (P-450AROM) enzyme and its messenger RNA (mRNA) in medial basal 
      hypothalamic (MBH) and preoptic area (POA) tissue during late fetal and perinatal 
      development of the rat. Aromatase activity in the MBH-POA was negligible before 
      gestational day (GD) 16 (< 0.1 pmol/h/mg protein), increased over 10-fold at GD 
      17 and continued to increase (over 5-fold) to peak levels at GD 19 (> 5.0 
      pmol/h/mg protein), and then declined to low levels at GD 22 and 2 days 
      post-birth (approximately 1 pmol/h/mg protein). The profile of P-450AROM mRNA in 
      the MBH-POA tissue was characterized by a predominant 2.7 kilobase (kb) mRNA 
      species, similar in size to the largest functional P-450AROM mRNA observed in 
      adult rat ovarian tissue. At GD 15, the P-450AROM mRNA was undetectable; low but 
      detectable levels were seen at GD 17, the abundance increased at later time 
      points and remained at peak levels on GDs 18 through 20, decreased slightly by GD 
      22, and then declined further by 2 days post-birth. The developmental increase in 
      P-450AROM mRNA levels correlated with the ascending pattern of enzyme activity 
      before GD 19, but the marked decrease in enzyme activity seen after GD 19 was not 
      accompanied by a corresponding decline in mRNA levels.(ABSTRACT TRUNCATED AT 250 
      WORDS)
FAU - Lephart, E D
AU  - Lephart ED
AD  - Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of 
      Texas, Southwestern Medical Center, Dallas 75235.
FAU - Simpson, E R
AU  - Simpson ER
FAU - McPhaul, M J
AU  - McPhaul MJ
FAU - Kilgore, M W
AU  - Kilgore MW
FAU - Wilson, J D
AU  - Wilson JD
FAU - Ojeda, S R
AU  - Ojeda SR
LA  - eng
GR  - AG08174/AG/NIA NIH HHS/United States
GR  - HD-09988/HD/NICHD NIH HHS/United States
GR  - HD-25123/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (DNA Probes)
RN  - 0 (Molecular Probes)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Messenger)
RN  - EC 1.14.14.1 (Aromatase)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/growth & development/*metabolism
MH  - Aromatase/*genetics
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Brain/embryology/enzymology/*physiology
MH  - DNA Probes
MH  - Embryonic and Fetal Development/physiology
MH  - Female
MH  - Hypothalamus, Middle/enzymology/metabolism
MH  - Male
MH  - Molecular Probes
MH  - Molecular Sequence Data
MH  - Preoptic Area/enzymology/metabolism
MH  - RNA Probes
MH  - RNA, Messenger/*metabolism
MH  - Rats
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 0169-328X(92)90224-Y [pii]
AID - 10.1016/0169-328x(92)90224-y [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1992 Dec;16(3-4):187-92. doi: 
      10.1016/0169-328x(92)90224-y.