PMID- 1337994
OWN - NLM
STAT- MEDLINE
DCOM- 19930408
LR  - 20151119
IS  - 0965-0407 (Print)
IS  - 0965-0407 (Linking)
VI  - 4
IP  - 10
DP  - 1992
TI  - Expression of neuroendocrine and epithelial markers in an adherent subline 
      derived from a classic small cell lung cancer cell line.
PG  - 419-29
AB  - Small cell lung cancer (SCLC) cell lines usually grow as floating aggregates, in 
      contrast to the adherent monolayers formed by non small cell lung cancer (NSCLC). 
      Induction of an adherent phenotype by a variety of methods has been the subject 
      of a number of recent publications. In this study, cultivation of the classic 
      SCLC cell line, NCI-H69, on a substratum provided by the pretreatment of tissue 
      culture dishes with medium conditioned by the growth of a well differentiated 
      squamous carcinoma cell line, HN5, induced an adherent phenotype with a variety 
      of epithelioid morphologies, commencing within 24 hr of plating. From such 
      cultures an adherent subline, H69A, has been established, which differs in its 
      growth, morphological characteristics, and immunocytochemical marker expression 
      from the parent NCI-H69 cells, and in its marker expression from other adherent 
      SCLC cell lines. H69A retained expression of neural cell adhesion molecule (NCAM) 
      and the neuroendocrine markers neuron specific enoclase, chromogranin A, and 
      synaptophysin, but showed diminished expression of the epithelial cell surface 
      markers AUA1, Ber-EP4, epithelial membrane antigen (EMA), and desmosomal protein. 
      Compared to NCI-H69 cells, the amounts of cytokeratin 18 detected were elevated, 
      while those of cytokeratin 19 were diminished in H69A cells. Focal expression of 
      cytokeratin 4 was found in some H69A cells, indicative of a capacity for partial 
      squamous differentiation. The expression of the cell surface glycoproteins 
      detected by AUA1 and Ber-EP4 was reduced throughout cultivation of the H69A 
      subline, while that of EMA and desmosomal protein was further diminished with 
      continued passage. Changes in the expression of these markers and NCAM were 
      evident in NCI-H69 cells grown on an HN5-derived substratum.
FAU - Walker, C
AU  - Walker C
AD  - Clatterbridge Cancer Research Trust, J. K. Douglas Cancer Research Laboratory, 
      Clatterbridge Hospital, Bebington, Merseyside, UK.
FAU - Wright-Perkins, S
AU  - Wright-Perkins S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Oncol Res
JT  - Oncology research
JID - 9208097
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Antigens, Surface)
RN  - 0 (Biomarkers)
RN  - 0 (Intermediate Filament Proteins)
SB  - IM
MH  - Antigens, Differentiation/analysis
MH  - Antigens, Neoplasm/analysis
MH  - Antigens, Surface/analysis
MH  - Biomarkers/*analysis
MH  - Carcinoma, Small Cell/immunology/*metabolism/*pathology
MH  - Epithelium/physiology
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Intermediate Filament Proteins/analysis
MH  - Lung Neoplasms/immunology/*metabolism/*pathology
MH  - Neurosecretory Systems/physiology
MH  - Tumor Cells, Cultured
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
PST - ppublish
SO  - Oncol Res. 1992;4(10):419-29.