PMID- 1338700
OWN - NLM
STAT- MEDLINE
DCOM- 19930528
LR  - 20061115
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 72
IP  - 11
DP  - 1992 Nov
TI  - [Hepatitis B virus infection in monocytes and their functional changes in 
      patients with chronic hepatitis B].
PG  - 651-4, 701
AB  - We detected the HBV DNA in peripheral blood monocytes of 70 patients with chronic 
      hepatitis B and 20 healthy controls by polymerase chain reaction (PCR) and 
      Southern transfer hybridization technique, the HLA-DR expression on monocytic 
      membrane and tumor necrosis factor-alpha (TNF alpha) content and interleukin 1 
      (IL 1) activity in their monocytic culture supernatant were also measured 
      respectively by indirect immunofluorescence technique, ELISA, and thymocytic 
      multiplication assay. HBV DNA sequences were detected in monocytes of 42(60%) of 
      the 70 patients. The percentage of monocyte-expressing HLADR and the TNF alpha 
      content in monocyte culture supernatant were significantly elevated in patients 
      with HBV DNA-positive monocytes compared with those patients with 
      HBV-DNA-negative monocytes. The percentage of monocyte-expressing HLA-DA and the 
      level of TNF alpha produced by monocytes correlated markedly with the intensity 
      of HBV DNA-positive signal in monocytes. These data suggested that HBV infection 
      in monocytes might cause a series of functional changes in these cells.
FAU - Guang, L X
AU  - Guang LX
AD  - Department of Infectious Disease, Southwest Hospital, Third Military Medical 
      College, Chongqing.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (DNA, Viral)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - DNA, Viral/blood
MH  - Female
MH  - HLA-DR Antigens/blood
MH  - Hepatitis B/*immunology/microbiology
MH  - Hepatitis B virus/genetics/*isolation & purification
MH  - Hepatitis, Chronic/immunology/microbiology
MH  - Humans
MH  - Interleukin-1/blood
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*immunology/*microbiology
MH  - Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 1992 Nov;72(11):651-4, 701.