PMID- 1339267
OWN - NLM
STAT- MEDLINE
DCOM- 19920218
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 182
IP  - 1
DP  - 1992 Jan 15
TI  - Characterization of the 5'-flanking region of the human brain-derived 
      neurotrophic factor gene.
PG  - 325-32
AB  - The 5'-flanking region of the human brain-derived neurotrophic factor (BDNF) gene 
      was isolated from a human placental genomic library using the cDNA fragment for 
      the 5'-noncoding region of human BDNF as a probe. A 3.2 Kbp genomic fragment 
      containing the 5'-flanking region, the first exon and a portion of the first 
      intron was isolated and sequenced. The transcriptional initiation site, 
      identified by S1 nuclease mapping, was located 26 bp downstream from the 
      TATA-like sequence. Several expression plasmids, in which the BDNF promoter 
      regions were fused to the chloramphenicol acetyltransferase (CAT) gene, were 
      constructed. Transient expression in human glioma Hs683 cells demonstrated that a 
      fragment of about 0.5 Kbp from the transcriptional initiation site was sufficient 
      for promoter activity.
FAU - Shintani, A
AU  - Shintani A
AD  - Biotechnology Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, 
      Japan.
FAU - Ono, Y
AU  - Ono Y
FAU - Kaisho, Y
AU  - Kaisho Y
FAU - Igarashi, K
AU  - Igarashi K
LA  - eng
SI  - GENBANK/D10271
SI  - GENBANK/D10272
SI  - GENBANK/D10273
SI  - GENBANK/D10274
SI  - GENBANK/D10275
SI  - GENBANK/D10276
SI  - GENBANK/M84512
SI  - GENBANK/M84513
SI  - GENBANK/S75889
SI  - GENBANK/S75896
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor
MH  - Chloramphenicol O-Acetyltransferase/genetics/metabolism
MH  - Female
MH  - Gene Library
MH  - Glioma
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/*genetics
MH  - Nerve Tissue Proteins/*genetics
MH  - Oligodeoxyribonucleotides
MH  - Placenta/physiology
MH  - Pregnancy
MH  - *Promoter Regions, Genetic
MH  - Recombinant Fusion Proteins/metabolism
MH  - TATA Box
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 1992/01/15 00:00
MHDA- 1992/01/15 00:01
CRDT- 1992/01/15 00:00
PHST- 1992/01/15 00:00 [pubmed]
PHST- 1992/01/15 00:01 [medline]
PHST- 1992/01/15 00:00 [entrez]
AID - S0006-291X(05)80148-2 [pii]
AID - 10.1016/s0006-291x(05)80148-2 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1992 Jan 15;182(1):325-32. doi: 
      10.1016/s0006-291x(05)80148-2.