PMID- 1340155
OWN - NLM
STAT- MEDLINE
DCOM- 19931022
LR  - 20190616
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 660
DP  - 1992 Oct 28
TI  - Variables affecting antisense RNA inhibition of gene expression.
PG  - 45-56
AB  - We have sought to determine what variables affect the extent of inhibition of 
      gene expression by stable nuclear-derived antisense RNAs. Myosin heavy chain gene 
      II (MHCII) expression in Dictyostelium was used as a model system, because 
      previous results have shown that nearly complete inhibition of expression can be 
      achieved under appropriate conditions. Various fragments of the myosin gene were 
      inserted into several transformation vectors in both sense and antisense 
      orientation, and the effects on expression of protein and RNA from the endogenous 
      MHC II gene were assayed. The results indicate that the critical factor was the 
      particular fragment of the gene used to produce the antisense RNA. Some fragments 
      produced complete inhibition of expression, whereas others gave only slight 
      inhibition. The fragments that produced the greatest inhibition were from the 
      tail region of the gene. In addition, cells were capable of overcoming the 
      inhibition while still expressing the antisense RNA. We hypothesize that the 
      three-dimensional topology of the antisense and sense RNAs determines their 
      accessibility for interstrand hybridization. Transformation with sense fragments 
      of the myosin gene also caused inhibition of the endogenous myosin gene. The 
      inhibition seen with sense expression is not as dramatic as that for antisense, 
      but it had the same phenotypic consequences for the cells. This phenomenon, which 
      has now been documented in other systems, may be mechanistically similar to 
      inhibition by antisense RNA in our system.
FAU - Scherczinger, C A
AU  - Scherczinger CA
AD  - Department of Molecular and Cell Biology, University of Connecticut, Storrs 
      06269.
FAU - Yates, A A
AU  - Yates AA
FAU - Knecht, D A
AU  - Knecht DA
LA  - eng
GR  - GM40599/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (RNA, Antisense)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
MH  - Animals
MH  - Dictyostelium/*drug effects
MH  - Gene Expression Regulation/*drug effects
MH  - Genes, Protozoan/*genetics
MH  - Genetic Vectors
MH  - Myosins/*genetics
MH  - RNA, Antisense/*pharmacology
MH  - Transformation, Genetic
EDAT- 1992/10/28 00:00
MHDA- 1992/10/28 00:01
CRDT- 1992/10/28 00:00
PHST- 1992/10/28 00:00 [pubmed]
PHST- 1992/10/28 00:01 [medline]
PHST- 1992/10/28 00:00 [entrez]
AID - 10.1111/j.1749-6632.1992.tb21056.x [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 1992 Oct 28;660:45-56. doi: 10.1111/j.1749-6632.1992.tb21056.x.