PMID- 1347247
OWN - NLM
STAT- MEDLINE
DCOM- 19920409
LR  - 20190719
IS  - 0361-9230 (Print)
IS  - 0361-9230 (Linking)
VI  - 28
IP  - 1
DP  - 1992 Jan
TI  - Lack of serotonin neurotoxicity after intraraphe microinjection of 
      (+)-3,4-methylenedioxymethamphetamine (MDMA).
PG  - 115-9
AB  - Systemic administration of 3,4-methylenedioxymethamphetamine (MDMA) produces 
      depletions of serotonin (5-HT) and its primary metabolite, 5-hydroxyindoleacetic 
      acid (5-HIAA), decreases 5-HT reuptake sites and diminishes tryptophan 
      hydroxylase activity in various forebrain regions. MDMA has been shown to be 
      neurotoxic to the fine fibers originating from dorsal raphe (DR) 5-HT neurons but 
      not the beaded fibers from the median raphe (MR) nucleus. In the present 
      experiment, MDMA was microinjected directly into the DR or MR to determine 
      whether differential neurotoxicity developed in the DR versus MR fiber systems as 
      measured by 5-HT levels and immunocytochemistry. Two weeks following stereotaxic 
      injection with either vehicle or (+)MDMA (50 micrograms base in 2 microliters) 
      into the DR or MR, rat brains were assayed for 5-HT and catecholamine content or 
      5-HT immunocytochemistry. HPLC analysis revealed no significant changes in 
      monoamine or metabolite concentrations in the hippocampus and striatum of rats 
      administered intra-DR or -MR (+)MDMA. Raphe sections stained for 5-HT also did 
      not reveal any apparent neurotoxicity. A single cerebral injection of (+)MDMA 
      does not produce neurotoxicity to 5-HT neuronal systems originating in the raphe, 
      although neurotoxicity of multiple MDMA injections into these raphe nuclei cannot 
      be ruled out.
FAU - Paris, J M
AU  - Paris JM
AD  - Department of Pharmacology and Toxicology, University of Texas Medical Branch, 
      Galveston 77550.
FAU - Cunningham, K A
AU  - Cunningham KA
LA  - eng
GR  - DA05381/DA/NIDA NIH HHS/United States
GR  - DA05708/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 333DO1RDJY (Serotonin)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*analogs & derivatives/toxicity
MH  - Animals
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Microinjections
MH  - N-Methyl-3,4-methylenedioxyamphetamine
MH  - *Raphe Nuclei
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Serotonin/*metabolism
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 0361-9230(92)90237-R [pii]
AID - 10.1016/0361-9230(92)90237-r [doi]
PST - ppublish
SO  - Brain Res Bull. 1992 Jan;28(1):115-9. doi: 10.1016/0361-9230(92)90237-r.