PMID- 1348363
OWN - NLM
STAT- MEDLINE
DCOM- 19920506
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 89
IP  - 7
DP  - 1992 Apr 1
TI  - Expression of alternatively spliced human T-lymphotropic virus type I pX mRNA in 
      infected cell lines and in primary uncultured cells from patients with adult 
      T-cell leukemia/lymphoma and healthy carriers.
PG  - 3005-9
AB  - Although human T-cell lymphotropic virus type I (HTLV-I) is the etiologic agent 
      of adult T-cell leukemia/lymphoma (ATL), the role of viral gene expression in the 
      progression to and maintenance of the leukemic state in vivo is unclear because 
      of the inability of most previous studies to readily detect HTLV-I RNA in 
      infected individuals. By using the reverse transcriptase-polymerase chain 
      reaction, we detected spliced messages for the HTLV-I pX regulatory genes in 
      primary uncultured cells from ATL patients and healthy asymptomatic carriers. In 
      addition to the expected doubly spliced pX message, three alternatively spliced 
      mRNAs were demonstrated (pX delta 17, pX-p21rex, and pX-orfII mRNAs, where orf = 
      open reading frame). The same splice sites were shown in the messages from 
      uncultured ATL cells and from the HTLV-I-producing C10/MJ cell line. 
      Alternatively spliced pX mRNAs have the potential to code for known and putative 
      pX gene products. Among the transcripts is a monocistronic mRNA likely to code 
      for p21rex (pX-p21rex mRNA). Since alternative splicing of HTLV-I pX mRNA can be 
      found in primary uncultured cells, it is likely to have a functional significance 
      in vivo. This suggests possible roles for HTLV-I gene expression in the 
      progression to and maintenance of ATL, as well as in the phase preceding it.
FAU - Berneman, Z N
AU  - Berneman ZN
AD  - Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes 
      of Health, Bethesda, MD 20892.
FAU - Gartenhaus, R B
AU  - Gartenhaus RB
FAU - Reitz, M S Jr
AU  - Reitz MS Jr
FAU - Blattner, W A
AU  - Blattner WA
FAU - Manns, A
AU  - Manns A
FAU - Hanchard, B
AU  - Hanchard B
FAU - Ikehara, O
AU  - Ikehara O
FAU - Gallo, R C
AU  - Gallo RC
FAU - Klotman, M E
AU  - Klotman ME
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Deltaretrovirus Infections/genetics
MH  - Gene Expression
MH  - *Genes, pX
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Humans
MH  - In Vitro Techniques
MH  - Leukemia-Lymphoma, Adult T-Cell/*genetics
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides/chemistry
MH  - Polymerase Chain Reaction
MH  - RNA Splicing
MH  - RNA, Messenger/genetics
PMC - PMC48792
EDAT- 1992/04/11 19:15
MHDA- 2001/03/28 10:01
PMCR- 1992/10/01
CRDT- 1992/04/11 19:15
PHST- 1992/04/11 19:15 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1992/04/11 19:15 [entrez]
PHST- 1992/10/01 00:00 [pmc-release]
AID - 10.1073/pnas.89.7.3005 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):3005-9. doi: 10.1073/pnas.89.7.3005.