PMID- 1348547
OWN - NLM
STAT- MEDLINE
DCOM- 19920514
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 66
IP  - 5
DP  - 1992 May
TI  - Establishment and functional characterization of human herpesvirus 6-specific 
      CD4+ human T-cell clones.
PG  - 2773-9
AB  - In order to clarify the protective immune responses against a newly identified 
      herpesvirus, human herpesvirus 6 (HHV-6), we established HHV-6-specific human 
      T-cell clones and examined their functional properties. Five CD3+CD4+CD8- T-cell 
      clones, which proliferated in response to stimulation with two different strains 
      of HHV-6 in the presence of autologous antigen-presenting cells but not with 
      herpes simplex virus type 1 or human cytomegalovirus, were established from 
      peripheral blood lymphocytes of a healthy individual. The proliferative response 
      of all T-cell clones to HHV-6 antigen was inhibited by addition of anti-HLA-DR 
      monoclonal antibody, indicating that these clones were human leukocyte antigen 
      (HLA) class II DR restricted. Of the five clones, two lysed HHV-6-infected 
      autologous lymphoblasts, but not HHV-6-infected allogeneic cells or natural 
      killer-sensitive K562 cells (group 1); one showed cytotoxicity against 
      HHV-6-infected autologous lymphoblasts as well as HHV-6-infected allogeneic cells 
      and K562 cells (group 2); and the remaining two showed no cytotoxic activity 
      (group 3). The cytotoxic activity of group 1 was inhibited by addition of 
      anti-HLA-DR monoclonal antibody to the culture, whereas this monoclonal antibody 
      had no effect on the cytotoxicity of group 2 and did not induce the cytotoxicity 
      of group 3. Perforin, which is one of the mediators of cytotoxicity, was 
      abundantly expressed in group 1 and 2 clones. Moreover, all groups of clones 
      produced gamma interferon after culture with antigen-presenting cells followed by 
      HHV-6 antigen stimulation. These results suggest that HHV-6-specific CD4+ T cells 
      have heterogeneous functions.
FAU - Yakushijin, Y
AU  - Yakushijin Y
AD  - First Department of Internal Medicine, Ehime University School of Medicine, 
      Japan.
FAU - Yasukawa, M
AU  - Yasukawa M
FAU - Kobayashi, Y
AU  - Kobayashi Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Viral)
RN  - 0 (HLA Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Pore Forming Cytotoxic Proteins)
RN  - 126465-35-8 (Perforin)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, Viral/immunology
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - *Clone Cells
MH  - Cytomegalovirus Infections/immunology
MH  - Cytotoxicity, Immunologic
MH  - HLA Antigens/immunology
MH  - Herpesviridae Infections/*immunology
MH  - Herpesvirus 6, Human/*immunology
MH  - Humans
MH  - Interferon-gamma/biosynthesis
MH  - Lymphocyte Activation/drug effects
MH  - *Membrane Glycoproteins
MH  - Membrane Proteins/biosynthesis
MH  - Perforin
MH  - Pore Forming Cytotoxic Proteins
PMC - PMC241033
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
PMCR- 1992/05/01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
PHST- 1992/05/01 00:00 [pmc-release]
AID - 10.1128/JVI.66.5.2773-2779.1992 [doi]
PST - ppublish
SO  - J Virol. 1992 May;66(5):2773-9. doi: 10.1128/JVI.66.5.2773-2779.1992.