PMID- 1349618
OWN - NLM
STAT- MEDLINE
DCOM- 19920609
LR  - 20171116
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 148
IP  - 10
DP  - 1992 May 15
TI  - Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, 
      CDw17, and CDw65).
PG  - 3221-9
AB  - The leukocyte carbohydrate (CHO) Ag CD15, sialyl-CD15, and CDw65 have recently 
      been found to function as ligands for CD62 and ELAM-1 cell adhesion molecules on 
      platelets and endothelium, respectively. Cell adhesion ligands also may act as 
      receptors capable of signal transduction. We therefore investigated the 
      possibility that these CHO Ag and CDw17, a glycolipid Ag whose expression is 
      regulated by leukocyte activation, may have receptor-like characteristics. The 
      effects of antibody cross-linking of CHO Ag on phagocyte activation were measured 
      by using flow cytometry and fluorescent indicators for cytoplasmic calcium ions, 
      oxidative burst, and the granule-associated proteins CD11b and CD67. 
      Cross-linking of CD15, sialyl-CD15, CDw65, or CDw17 induced a moderate release of 
      calcium ions into the cytoplasm of granulocytes, a strong activation of oxidative 
      burst, and a low up-regulation of CD11b and CD67 compared to the effects of 
      treatment with 4 microM FMLP. The results suggest a role for CHO Ag in leukocyte 
      signal transduction and support the view that these molecules are involved in 
      phagocyte activation.
FAU - Lund-Johansen, F
AU  - Lund-Johansen F
AD  - Department of Pathology, Gade Institute, University of Bergen, Haukeland 
      Hospital, Norway.
FAU - Olweus, J
AU  - Olweus J
FAU - Horejsi, V
AU  - Horejsi V
FAU - Skubitz, K M
AU  - Skubitz KM
FAU - Thompson, J S
AU  - Thompson JS
FAU - Vilella, R
AU  - Vilella R
FAU - Symington, F W
AU  - Symington FW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Lewis X Antigen)
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgG)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens, CD/*physiology
MH  - Antigens, Differentiation/physiology
MH  - *Antigens, Neoplasm
MH  - Calcium/metabolism
MH  - *Cell Adhesion Molecules
MH  - Cells, Cultured
MH  - Cytoplasm/metabolism
MH  - Humans
MH  - Lewis X Antigen
MH  - Macrophage-1 Antigen/physiology
MH  - Membrane Glycoproteins/physiology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Phagocytes/*physiology
MH  - Receptors, Fc/physiology
MH  - Receptors, IgG
MH  - Respiratory Burst
EDAT- 1992/05/15 00:00
MHDA- 1992/05/15 00:01
CRDT- 1992/05/15 00:00
PHST- 1992/05/15 00:00 [pubmed]
PHST- 1992/05/15 00:01 [medline]
PHST- 1992/05/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1992 May 15;148(10):3221-9.