PMID- 1353776
OWN - NLM
STAT- MEDLINE
DCOM- 19920908
LR  - 20190907
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 62
IP  - 1
DP  - 1992 Jul
TI  - Rapid translocation frequency analysis in humans decades after exposure to 
      ionizing radiation.
PG  - 53-63
AB  - This paper presents an analysis of the utility of fluorescence in situ 
      hybridization (FISH) with whole-chromosome probes for measurement of the genomic 
      frequency of translocations found in the peripheral blood of individuals exposed 
      to ionizing radiation. First, we derive the equation: Fp = 2.05fp(1-fp)FG, 
      relating the translocation frequency, Fp, measured using FISH to the genomic 
      translocation frequency, FG, where fp is the fraction of the genome covered by 
      the composite probe. We demonstrate the validity of this equation by showing 
      that: (a) translocation detection efficiency predicted by the equation is 
      consistent with experimental data as fp is changed; (b) translocation frequency 
      dose-response curves measured in vitro using FISH agree well with dicentric 
      frequency dose-response curves measured in vitro using conventional cytogenetic 
      procedures; and (c) the genomic translocation frequencies estimated from FISH 
      measurements for 20 Hiroshima A-bomb survivors and four workers exposed to 
      ionizing radiation during the Y-12 criticality accident are approximately the 
      same as the translocation frequencies measured using G-banding. We also show that 
      translocation frequency dose response curves estimated using FISH are similar for 
      Hiroshima A-bomb survivors and for first division lymphocytes irradiated in 
      vitro. We conclude with a discussion of the potential utility of translocation 
      frequency analysis for assessment of the level of acute radiation exposure 
      independent of the time between analysis and exposure.
FAU - Lucas, J N
AU  - Lucas JN
AD  - Lawrence Livermore National Laboratory, University of California, Livermore 
      94550.
FAU - Awa, A
AU  - Awa A
FAU - Straume, T
AU  - Straume T
FAU - Poggensee, M
AU  - Poggensee M
FAU - Kodama, Y
AU  - Kodama Y
FAU - Nakano, M
AU  - Nakano M
FAU - Ohtaki, K
AU  - Ohtaki K
FAU - Weier, H U
AU  - Weier HU
FAU - Pinkel, D
AU  - Pinkel D
FAU - Gray, J
AU  - Gray J
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
SB  - IM
MH  - Accidents
MH  - Blood/radiation effects
MH  - Fluorescence
MH  - Humans
MH  - Male
MH  - Nuclear Warfare
MH  - Nucleic Acid Hybridization
MH  - *Radiation Genetics
MH  - Time Factors
MH  - *Translocation, Genetic
EDAT- 1992/07/01 00:00
MHDA- 1992/07/01 00:01
CRDT- 1992/07/01 00:00
PHST- 1992/07/01 00:00 [pubmed]
PHST- 1992/07/01 00:01 [medline]
PHST- 1992/07/01 00:00 [entrez]
AID - 10.1080/09553009214551821 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1992 Jul;62(1):53-63. doi: 10.1080/09553009214551821.