PMID- 1354613
OWN - NLM
STAT- MEDLINE
DCOM- 19920922
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 11
IP  - 9
DP  - 1992 Sep
TI  - Transcriptional repression of band 3 and CAII in v-erbA transformed erythroblasts 
      accounts for an important part of the leukaemic phenotype.
PG  - 3355-65
AB  - The v-erbA oncogene confers two prominent properties on transformed 
      erythroblasts: a block of spontaneous differentiation and tolerance to wide 
      variations in the pH or ionic strength of culture medium. V-erbA acts as a 
      constitutive repressor of erythrocyte-specific gene transcription, arresting the 
      expression of at least three different erythroid genes: the erythrocyte anion 
      transporter (band 3), carbonic anhydrase II (CAII) and delta-aminolevulinate 
      synthase (ALA-S). To test whether or not the v-erbA induced repression of these 
      genes is causally related to the v-erbA induced leukaemic phenotype, we have 
      reintroduced the genes for band 3 or CAII into transformed erythroblasts via 
      retrovirus vectors. We show here that such erythroblasts, expressing v-erbA, 
      require the same narrow range of medium pH and ion concentration for growth as do 
      transformed erythroblasts lacking v-erbA, i.e. the v-erbA induced tolerance to pH 
      variation was abrogated. The v-erbA induced differentiation block, however, 
      remained unaffected by the re-expression of band 3 and was only slightly affected 
      by the re-expression of CAII. Our experiments show that the two v-erbA-related 
      'erythroblast transformation parameters' are separable: suppression of band 3 and 
      CAII accounts for one parameter (pH/ion tolerance), while the second parameter 
      (differentiation block) must involve v-erbA regulation of a different set of 
      target genes.
FAU - Fuerstenberg, S
AU  - Fuerstenberg S
AD  - Department of Molecular Biology, CMB, Karolinska Institute, Stockholm, Sweden.
FAU - Leitner, I
AU  - Leitner I
FAU - Schroeder, C
AU  - Schroeder C
FAU - Schwarz, H
AU  - Schwarz H
FAU - Vennstrom, B
AU  - Vennstrom B
FAU - Beug, H
AU  - Beug H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Anion Exchange Protein 1, Erythrocyte)
RN  - 0 (Anion Transport Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Oncogene Proteins v-erbA)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - EC 2.3.1.37 (5-Aminolevulinate Synthetase)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - 5-Aminolevulinate Synthetase/genetics
MH  - Alpharetrovirus/genetics
MH  - Animals
MH  - Anion Exchange Protein 1, Erythrocyte/*genetics
MH  - Anion Transport Proteins
MH  - Base Sequence
MH  - Carbonic Anhydrases/*genetics
MH  - Carrier Proteins/genetics
MH  - Cell Differentiation
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Cell Transformation, Viral/genetics
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Erythroblasts/metabolism/microbiology
MH  - Fibroblasts/microbiology
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genetic Vectors
MH  - Hydrogen-Ion Concentration
MH  - Leukemia, Experimental/enzymology/*genetics
MH  - Molecular Sequence Data
MH  - Oncogene Proteins v-erbA
MH  - Phenotype
MH  - Retroviridae Proteins, Oncogenic/*genetics
PMC - PMC556870
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
PMCR- 1993/09/01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
PHST- 1993/09/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1992.tb05414.x [doi]
PST - ppublish
SO  - EMBO J. 1992 Sep;11(9):3355-65. doi: 10.1002/j.1460-2075.1992.tb05414.x.