PMID- 1357059
OWN - NLM
STAT- MEDLINE
DCOM- 19921029
LR  - 20190907
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 62
IP  - 4
DP  - 1992 Oct
TI  - Transformation and radiosensitivity of human diploid skin fibroblasts transfected 
      with SV40 T-antigen mutants defective in RB and P53 binding domains.
PG  - 461-8
AB  - A series of human diploid fibroblast cell clones were developed by DNA 
      transfection with either wild-type SV40 T-antigen (SV40 T) or T-antigen mutants 
      defective in its various functional domains. Cell clones expressing the wild-type 
      SV40 T were significantly radioresistant as compared with clones transfected with 
      the neo gene only (D0 = 192 +/- 13 vs 127 +/- 19). This radioresistance persisted 
      in post-crisis, immortalized cell lines. A series of mutants with point or 
      deletion mutations within each functionally active domain of SV40 T were also 
      examined for their ability to alter radiosensitivity and induce morphological 
      transformation. Cell clones transfected with T-antigen mutants defective in 
      nuclear localization or origin binding showed increased radioresistance similar 
      to clones transfected with wild-type T-antigen, and expressed morphological 
      changes characteristic of SV40 T-transfected cells. A retinoblastoma 
      susceptibility gene (RB) binding defective mutant showed moderately increased 
      radioresistance (D0 = 174 +/- 10). However, cell clones transfected with three 
      different p53 binding defective mutants showed no change in radiosensitivity (D0 
      = 132 +/- 5) as compared with neo gene transfected controls. Transfection with 
      T-antigen mutants defective in either the RB or p53 binding domain yielded no 
      morphological alterations characteristic of transformation. These data suggest 
      that the SV40 T/p53 complex may be of importance in the radioresistance 
      phenotype.
FAU - Su, L N
AU  - Su LN
AD  - Laboratory of Radiobiology, Harvard School of Public Health, Boston, 
      Massachusetts 02115.
FAU - Little, J B
AU  - Little JB
LA  - eng
GR  - CA-47542/CA/NCI NIH HHS/United States
GR  - ES-00002/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (Antigens, Polyomavirus Transforming)
SB  - IM
GS  - RB
GS  - p53
MH  - Antigens, Polyomavirus Transforming/chemistry/*genetics
MH  - Cell Adhesion
MH  - Cell Line
MH  - Cell Survival
MH  - Cell Transformation, Viral/*genetics/radiation effects
MH  - Colony-Forming Units Assay
MH  - Diploidy
MH  - Fibroblasts
MH  - *Genes, Retinoblastoma
MH  - *Genes, p53
MH  - Humans
MH  - Infant, Newborn
MH  - Male
MH  - Mutation
MH  - *Radiation Tolerance
MH  - Skin/cytology
MH  - *Transfection
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
AID - N5BWX68C2C26QHP4 [pii]
AID - 10.1080/09553009214552341 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1992 Oct;62(4):461-8. doi: 10.1080/09553009214552341.