PMID- 1358626
OWN - NLM
STAT- MEDLINE
DCOM- 19921208
LR  - 20210114
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 22
IP  - 11
DP  - 1992 Nov
TI  - Human neutrophils release their major membrane sialoprotein, leukosialin (CD43), 
      during cell activation.
PG  - 3021-6
AB  - Leukosialin (CD43) is a sialic acid-rich molecule with a relative molecular mass 
      (M(r)) of 140,000 highly represented on polymorphonuclear neutrophils (PMN) and 
      on most leukocytes. One of its functions may be to prevent nonspecific 
      cell-to-cell interactions through negative charge repulsions. As tested by 
      immunofluorescence, neutrophil CD43 membrane expression was shown to decrease by 
      up to 80% upon cell activation by phorbol myristate acetate (10 ng/ml) or by 
      N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP; 10(-6) M) in the presence of 
      cytochalasin B. The kinetic of this decrease paralleled that of CD11b 
      up-regulation. FMLP alone, tumor necrosis factor (TNF-alpha), lipopolysaccharide 
      and granulocyte macrophage colony-stimulating factor had moderate or 
      insignificant effects, while inducing striking CD11b up-regulation. Cell priming 
      with TNF-alpha followed by FMLP stimulation resulted in up to 40% decrease of 
      CD43 expression. Anti-CD43 mAb immunoprecipitated three fragments of M(r) 
      130,000, 49,000 and 34,000 from the cell-free supernatant of activated 
      neutrophils, suggesting that CD43 is released from the membrane by proteolysis. 
      Indeed, the decrease in CD43 expression was inhibited by 
      phenylmethanesulfonylfluoride (PMSF). Homotypic aggregation of activated PMN was 
      also inhibited by PMSF and could result, at least in part, from the shedding of 
      CD43. The shedding of such a strongly anionic and major membrane protein should 
      drastically modify PMN surface charge and may allow previously hindered 
      interactions by exposing new adhesion molecules.
FAU - Rieu, P
AU  - Rieu P
AD  - INSERM U 90, Hopital Necker, Paris, France.
FAU - Porteu, F
AU  - Porteu F
FAU - Bessou, G
AU  - Bessou G
FAU - Lesavre, P
AU  - Lesavre P
FAU - Halbwachs-Mecarelli, L
AU  - Halbwachs-Mecarelli L
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens, CD)
RN  - 0 (CD11 Antigens)
RN  - 0 (CD18 Antigens)
RN  - 0 (Leukosialin)
RN  - 0 (SPN protein, human)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Spn protein, mouse)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Antigens, CD/analysis
MH  - CD11 Antigens
MH  - CD18 Antigens
MH  - Cell Aggregation
MH  - Cell Communication
MH  - Cells, Cultured
MH  - Down-Regulation
MH  - Humans
MH  - Leukosialin
MH  - Mice
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*physiology
MH  - Sialoglycoproteins/analysis/*physiology
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 10.1002/eji.1830221138 [doi]
PST - ppublish
SO  - Eur J Immunol. 1992 Nov;22(11):3021-6. doi: 10.1002/eji.1830221138.