PMID- 1358857
OWN - NLM
STAT- MEDLINE
DCOM- 19921217
LR  - 20111117
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 33
IP  - 12
DP  - 1992 Nov
TI  - Human leukocyte antigen serologic and DNA typing of Behcet's disease and its 
      primary association with B51.
PG  - 3332-40
AB  - Ninety Japanese patients with Behcet's disease (BD) were typed for human 
      leukocyte antigen (HLA)-DRB1, -DQA1-, -DQB1, and -DPB1 alleles by the polymerase 
      chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and for 
      HLA-A, -B, -C, -DR, and -DQ antigens by conventional serologic typing. Serologic 
      HLA typing showed a remarkably significant increase of HLA-B51 and a significant 
      decrease of HLA-DQw1 in the patients with BD, especially those with ocular 
      lesions including complete type, as compared with the control group (for B51, 
      chi-squared = 46.75, P corrected < 0.001, relative risk [RR] = 7.9; for DQw1, 
      chi-squared = 12.10, P corrected < 0.01, RR = 0.4). By PCR-RFLP genotyping, no 
      significant difference was revealed in any class II alleles between the patient 
      and the control groups in the corrected P value test, but P value analysis showed 
      the significantly high frequency of DRB1*0802 and the significantly low 
      frequencies of DQA1*0103, DQB1*0601, and DQB1*0501. No significant difference was 
      observed in any DPB1 alleles by either P value analysis. These results indicated 
      that the primary and primordial gene(s) responsible for the susceptibility to BD, 
      especially related to ocular lesions, were not located in the HLA class II gene 
      region but were in or very close to the HLA-B locus in the class I region. They 
      also suggested the possibility that BD was a symptom complex associated with some 
      independent diseases.
FAU - Mizuki, N
AU  - Mizuki N
AD  - Department of Ophthalmology, Yokohama City University School of Medicine, Japan.
FAU - Inoko, H
AU  - Inoko H
FAU - Mizuki, N
AU  - Mizuki N
FAU - Tanaka, H
AU  - Tanaka H
FAU - Kera, J
AU  - Kera J
FAU - Tsuiji, K
AU  - Tsuiji K
FAU - Ohno, S
AU  - Ohno S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B51 Antigen)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alleles
MH  - Behcet Syndrome/*genetics/immunology
MH  - DNA/*analysis
MH  - Genotype
MH  - HLA Antigens/*analysis/genetics
MH  - HLA-B Antigens/*analysis
MH  - HLA-B51 Antigen
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1992 Nov;33(12):3332-40.