PMID- 1359526
OWN - NLM
STAT- MEDLINE
DCOM- 19921217
LR  - 20190902
IS  - 0901-9928 (Print)
IS  - 0901-9928 (Linking)
VI  - 70
IP  - 6 Pt 1
DP  - 1992 Jun
TI  - Exogenous modification of nitrovasodilator-induced cyclic GMP formation in human 
      lymphocytes.
PG  - 463-7
AB  - The effects of exogenous guanosine 5'-triphosphate (GTP), guanosine 
      5'-(gamma-thio)triphosphate (GTP gamma S), cysteine and Trolox C, a water soluble 
      vitamin E analogue, were studied on basal and nitrovasodilator-induced cyclic GMP 
      formation in isolated human lymphocytes. Incubation of lymphocytes in the 
      presence of GTP (0.1 mM) and GTP gamma S (0.1 mM) increased cyclic GMP more than 
      twofold. SIN-1 and sodium nitroprusside dose-dependently increased cyclic GMP, 
      but nitroglycerin and sodium nitrite were ineffective. GTP and GTP gamma S 
      potentiated SIN-1 and sodium nitroprusside-induced cyclic GMP formation. In the 
      presence of GTP and GTP gamma S, nitroglycerin, but not sodium nitrite, was able 
      to increase lymphocyte cyclic GMP. Cysteine (1 mM) enhanced cyclic GMP formation 
      induced by sodium nitroprusside and nitroglycerin. Trolox C (0.1 mM) potentiated 
      SIN-1-induced cyclic GMP formation. These results indicate that exogenous GTP and 
      GTP gamma S enhance guanylate cyclase stimulation by spontaneous nitric oxide 
      releasers and nitroglycerin in lymphocytes. Cysteine, a redox-compound and Trolox 
      C, an antioxidant, have different effects on guanylate cyclase activation by 
      nitric oxide releasers, SIN-1 and sodium nitroprusside.
FAU - Vuorinen, P
AU  - Vuorinen P
AD  - Department of Biomedical Sciences, University of Tampere, Finland.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Pharmacol Toxicol
JT  - Pharmacology & toxicology
JID - 8702180
RN  - 0 (Chromans)
RN  - 0 (Vasodilator Agents)
RN  - 169D1260KM (Nitroprusside)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 5O5U71P6VQ (linsidomine)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - D46583G77X (Molsidomine)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
RN  - H2D2X058MU (Cyclic GMP)
RN  - K848JZ4886 (Cysteine)
RN  - S18UL9710X (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid)
SB  - IM
MH  - Chromans/pharmacology
MH  - Cyclic GMP/*biosynthesis/metabolism
MH  - Cysteine/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Enzyme Activation/drug effects
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Guanosine Triphosphate/*pharmacology
MH  - Guanylate Cyclase/drug effects/metabolism
MH  - Humans
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Lymphocytes/*drug effects/enzymology/metabolism
MH  - Molsidomine/analogs & derivatives/pharmacology
MH  - Nitroprusside/pharmacology
MH  - Vasodilator Agents/*pharmacology
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
AID - 10.1111/j.1600-0773.1992.tb00509.x [doi]
PST - ppublish
SO  - Pharmacol Toxicol. 1992 Jun;70(6 Pt 1):463-7. doi: 
      10.1111/j.1600-0773.1992.tb00509.x.