PMID- 1359744
OWN - NLM
STAT- MEDLINE
DCOM- 19921218
LR  - 20201126
IS  - 0065-4299 (Print)
IS  - 0065-4299 (Linking)
VI  - Spec No
DP  - 1992
TI  - Cytokines and platelet-activating factor in human inflamed colonic mucosa.
PG  - C32-6
AB  - Colonic biopsy specimens from patients with active ulcerative colitis and 
      controls were incubated for four hours in the presence or absence of calcium 
      ionophore or antihuman immunoglobulin E (IgE). Platelet-activating factor (PAF) 
      was determined in the tissue by aggregation assay after extraction with 80% 
      ethanol. PAF was not detected in normal mucosa, whereas A23187 and antihuman IgE 
      stimulated its activity: mean +/- SE, 43.2 +/- 8.6 and 33.0 +/- 6.1 pg/10 mg wet 
      weight, respectively. In active ulcerative colitis, A23187 and antihuman IgE 
      induced significantly higher stimulation of PAF synthesis compared to their 
      effects on normal mucosa. The enhanced stimulation of PAF induced by A23187 was 
      dose-dependently inhibited by sulphasalazine, 5-aminosalicylic acid and 
      prednisolone, but not by sulfapyridine. Colonic interleukin-1 content and release 
      during 24 h of culture were significantly higher in patients with active 
      ulcerative colitis and Crohn's disease compared to normal subjects. Prednisolone 
      significantly and dose-dependently inhibited interleukin-1 release. These results 
      suggest that colonic generation of PAF and interleukin-1 are elevated in patients 
      with inflammatory bowel disease and, thus, may have a role in its pathogenesis. 
      Pharmacological suppression of colonic PAF and interleukin-1 production may have 
      beneficial therapeutic effects.
FAU - Rachmilewitz, D
AU  - Rachmilewitz D
AD  - Department of Medicine, Hadassah University Hospital, Jerusalem, Israel.
FAU - Eliakim, R
AU  - Eliakim R
FAU - Simon, P
AU  - Simon P
FAU - Ligumsky, M
AU  - Ligumsky M
FAU - Karmeli, F
AU  - Karmeli F
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Agents Actions
JT  - Agents and actions
JID - 0213341
RN  - 0 (Aminosalicylic Acids)
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Imidazoles)
RN  - 0 (Interleukin-1)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (Thiazoles)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 3XC8GUZ6CB (Sulfasalazine)
RN  - 4Q81I59GXC (Mesalamine)
RN  - 9PHQ9Y1OLM (Prednisolone)
RN  - 9R6QDF1UO7 (6-(4-fluorophenyl)-2,3-dihydro-5-(4-pyridinyl)imidazo(2,1-b)thiazole)
SB  - IM
MH  - Aminosalicylic Acids/pharmacology
MH  - Anti-Inflammatory Agents, Non-Steroidal/pharmacology
MH  - Antibodies, Anti-Idiotypic/immunology
MH  - Calcimycin/pharmacology
MH  - Colitis, Ulcerative/drug therapy/*metabolism
MH  - Colon/drug effects/*metabolism
MH  - Crohn Disease/drug therapy/*metabolism
MH  - Humans
MH  - Imidazoles/pharmacology
MH  - Immunoglobulin E/immunology
MH  - Interleukin-1/*biosynthesis
MH  - Intestinal Mucosa/drug effects/*metabolism
MH  - Mesalamine
MH  - Organ Culture Techniques
MH  - Platelet Activating Factor/*biosynthesis
MH  - Prednisolone/pharmacology
MH  - Sulfasalazine/pharmacology
MH  - Thiazoles/pharmacology
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
PST - ppublish
SO  - Agents Actions. 1992;Spec No:C32-6.