PMID- 1366835
OWN - NLM
STAT- MEDLINE
DCOM- 19910410
LR  - 20131121
IS  - 8756-7938 (Print)
IS  - 1520-6033 (Linking)
VI  - 6
IP  - 6
DP  - 1990 Nov-Dec
TI  - Plant cell culture using a novel bioreactor: the magnetically stabilized 
      fluidized bed.
PG  - 452-7
AB  - A novel bioreactor using magnetically stabilized fluidized bed (MSFB) technology 
      has been developed that has certain advantages for cultivating cells 
      continuously. In this system, the cells are protected from shear and are 
      constrained to move through the fermenter in lock-step fashion by being 
      immobilized in calcium alginate beads. The MSFB permits good mass transfer, 
      minimizes particle collisions, and allows for the production of cells while 
      maintaining a controlled cell residence time. Details of the experimental system 
      are described. In addition, the experimental performance of an MSFB used to grow 
      plant cells in batch mode is compared to the results obtained in shake flask 
      culture.
FAU - Bramble, J L
AU  - Bramble JL
AD  - Department of Chemical Engineering, University of Pennsylvania, Philadelphia 
      19104.
FAU - Graves, D J
AU  - Graves DJ
FAU - Brodelius, P
AU  - Brodelius P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biotechnol Prog
JT  - Biotechnology progress
JID - 8506292
RN  - 0 (Coffee)
RN  - 0 (Oxides)
RN  - 3G6A5W338E (Caffeine)
RN  - E1UOL152H7 (Iron)
RN  - XM0M87F357 (Ferrosoferric Oxide)
MH  - Caffeine/metabolism
MH  - Cells, Cultured
MH  - Coffee/metabolism
MH  - Fermentation
MH  - Ferrosoferric Oxide
MH  - Iron
MH  - *Magnetics
MH  - Microspheres
MH  - Oxides
MH  - Plant Cells
MH  - Plants/*metabolism
EDAT- 1990/11/01 00:00
MHDA- 1990/11/01 00:01
CRDT- 1990/11/01 00:00
PHST- 1990/11/01 00:00 [pubmed]
PHST- 1990/11/01 00:01 [medline]
PHST- 1990/11/01 00:00 [entrez]
AID - 10.1021/bp00006a008 [doi]
PST - ppublish
SO  - Biotechnol Prog. 1990 Nov-Dec;6(6):452-7. doi: 10.1021/bp00006a008.