PMID- 13678262
OWN - NLM
STAT- MEDLINE
DCOM- 20031223
LR  - 20131121
IS  - 0027-769X (Print)
IS  - 0027-769X (Linking)
VI  - 47
IP  - 4
DP  - 2003 Aug
TI  - Determination of water-soluble vitamins in soft drinks and vitamin supplements 
      using capillary electrophoresis.
PG  - 243-7
AB  - A method for the determination of six water-soluble vitamins based on capillary 
      electrophoresis (CE) operated in micellar mode was developed. Thiamine 
      hydrochloride (vitamin B1), riboflavin (vitamin B2), pyridoxine hydrochloride 
      (vitamin B6), pantothenic acid (vitamin B5), nicotinamide (vitamin B3), and 
      cobalamin (Vitamin B12) could be separated in a single run. All CE parameters 
      such as buffer composition and operation temperature were optimized in order to 
      achieve better separation. Relative standard deviations (RSDs) of the described 
      method ranged from 1.08 to 3.68% (intra-day precision) and 1.26 to 3.35% 
      (inter-day precision). The method was then used for measuring various soft drinks 
      and vitamin supplements directly without any step of sample cleanup. The 
      determination of niacin was successful for all samples tested, reaching 
      recoveries near 100%. Riboflavin and pyridoxine were quantified successfully in 
      some but not all samples. Therefore, an evaluation on a case-by-case basis is 
      mandatory. When applicable, this method provides a fast, accurate, simple, and 
      inexpensive way to quantify selected vitamins, and is therefore well suited for 
      routine analysis in soft drink industry.
FAU - Schreiner, Matthias
AU  - Schreiner M
AD  - Department of Dairy Research and Microbiology, BOKU--University of Natural 
      Resources and Applied Life Sciences, Vienna, Austria. 
      matthias.schreiner@boku.ac.at
FAU - Razzazi, Ebrahim
AU  - Razzazi E
FAU - Luf, Wolfgang
AU  - Luf W
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Nahrung
JT  - Die Nahrung
JID - 0142530
RN  - 059QF0KO0R (Water)
RN  - 12001-76-2 (Vitamin B Complex)
SB  - IM
MH  - Beverages/*analysis
MH  - Dietary Supplements
MH  - *Electrophoresis, Capillary/methods/standards
MH  - Food, Fortified/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solubility
MH  - Vitamin B Complex/*analysis
MH  - Water/chemistry
EDAT- 2003/09/19 05:00
MHDA- 2003/12/24 05:00
CRDT- 2003/09/19 05:00
PHST- 2003/09/19 05:00 [pubmed]
PHST- 2003/12/24 05:00 [medline]
PHST- 2003/09/19 05:00 [entrez]
AID - 10.1002/food.200390057 [doi]
PST - ppublish
SO  - Nahrung. 2003 Aug;47(4):243-7. doi: 10.1002/food.200390057.