PMID- 13679050 OWN - NLM STAT- MEDLINE DCOM- 20031204 LR - 20190612 IS - 0006-291X (Print) IS - 0006-291X (Linking) VI - 309 IP - 4 DP - 2003 Oct 3 TI - Effects of prolonged delivery of brain-derived neurotrophic factor on the fate of neural stem cells transplanted into the developing rat retina. PG - 843-7 AB - One of the major roles of brain-derived neurotrophic factor (BDNF) is to promote the differentiation and support the survival of neurons in the central nervous system. The objective of the present study was to evaluate the effect of BDNF on the fate of adult rat hippocampus-derived neural stem cells (AHPCs) transplanted into the developing rat retina. Immunohistochemical analysis showed a significant increase in the ratio of grafted AHPCs stained for MAP2ab (P<0.05) and a marked decrease in the ratio of nestin-positive grafted cells in the slow-releasing BDNF group compared with the control group. The respective changes in the ratios of MAP5 and GFAP-positive grafted cells were comparable for the two groups. The results reported here suggest a potentially beneficial role for extended delivery of BDNF in the differentiation of grafted neural stem cells, which may lead to a novel modification of stem cell transplantation. FAU - Suzuki, Takuya AU - Suzuki T AD - Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan. FAU - Ooto, Sotaro AU - Ooto S FAU - Akagi, Tadamichi AU - Akagi T FAU - Amemiya, Kaori AU - Amemiya K FAU - Igarashi, Rie AU - Igarashi R FAU - Mizushima, Yutaka AU - Mizushima Y FAU - Takahashi, Masayo AU - Takahashi M LA - eng PT - Journal Article PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 RN - 0 (Brain-Derived Neurotrophic Factor) SB - IM MH - Animals MH - Animals, Newborn MH - Brain-Derived Neurotrophic Factor/*administration & dosage MH - Hippocampus/*cytology MH - Immunohistochemistry MH - Rats MH - Rats, Inbred F344 MH - Retina/*growth & development MH - *Stem Cell Transplantation EDAT- 2003/09/19 05:00 MHDA- 2003/12/05 05:00 CRDT- 2003/09/19 05:00 PHST- 2003/09/19 05:00 [pubmed] PHST- 2003/12/05 05:00 [medline] PHST- 2003/09/19 05:00 [entrez] AID - S0006291X03016760 [pii] AID - 10.1016/j.bbrc.2003.08.076 [doi] PST - ppublish SO - Biochem Biophys Res Commun. 2003 Oct 3;309(4):843-7. doi: 10.1016/j.bbrc.2003.08.076.