PMID- 13679140
OWN - NLM
STAT- MEDLINE
DCOM- 20040719
LR  - 20190823
IS  - 0378-5955 (Print)
IS  - 0378-5955 (Linking)
VI  - 183
IP  - 1-2
DP  - 2003 Sep
TI  - Expression of hypoxia-inducible factor-1 in the cochlea of newborn rats.
PG  - 73-83
AB  - Hypoxia/ischemia is a major pathogenetic factor in the development of hearing 
      loss. An important transcription factor involved in the signaling and adaptation 
      to hypoxia/ischemia is the hypoxia-inducible factor-1 (HIF-1). To study HIF-1 
      expression we used an in vitro hypoxia model of explant and dissociated cultures 
      of the stria vascularis, the organ of Corti with limbus and the modiolus from the 
      cochlea of 3-5-day-old Wistar rats. Hypoxia differentially increased HIF-1 
      activity as measured by a reporter gene. Twenty-four hour hypoxia increased HIF-1 
      activity 14.1+/-3.5-fold in the modiolus, 9.4+/-3.0-fold in the organ of Corti 
      with limbus, and 6.4+/-1.5-fold in the stria vascularis. The HIF-1alpha mRNA 
      level was measured by quantitative reverse transcription polymerase chain 
      reaction and showed a lower expression in the modiolus (1.3+/-0.2 pg/microg RNA) 
      than in both the organ of Corti with limbus and the stria vascularis 
      (2.7-3.2+/-1.3, P<0.01). Hypoxia had no effect on the HIF-1alpha mRNA levels. The 
      region-specific regulation of HIF-1 expression on the transcriptional and 
      posttranslational levels may expand the possibilities for adaptation of the 
      cochlea to hypoxia.
FAU - Gross, Johann
AU  - Gross J
AD  - Department of Otorhinolaryngology, University Hospital Charite, Spandauer Damm 
      130, Bldg 31, 14050 Berlin, Germany. johann.gross@charite.de
FAU - Rheinlander, Cornelia
AU  - Rheinlander C
FAU - Fuchs, Julia
AU  - Fuchs J
FAU - Mazurek, Birgit
AU  - Mazurek B
FAU - Machulik, Astrid
AU  - Machulik A
FAU - Andreeva, Nadezhda
AU  - Andreeva N
FAU - Kietzmann, T
AU  - Kietzmann T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Hear Res
JT  - Hearing research
JID - 7900445
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cochlea/cytology/*metabolism
MH  - DNA-Binding Proteins/*biosynthesis/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression
MH  - Genes, Reporter
MH  - Hearing Loss/etiology/*metabolism
MH  - Histocytochemistry
MH  - Hypoxia/complications/*metabolism
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Immunohistochemistry
MH  - Luciferases/metabolism
MH  - Nuclear Proteins/*biosynthesis/genetics
MH  - Organ Culture Techniques
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription Factors/analysis/biosynthesis/genetics
MH  - Transfection
EDAT- 2003/09/19 05:00
MHDA- 2004/07/20 05:00
CRDT- 2003/09/19 05:00
PHST- 2003/09/19 05:00 [pubmed]
PHST- 2004/07/20 05:00 [medline]
PHST- 2003/09/19 05:00 [entrez]
AID - S0378595503002223 [pii]
AID - 10.1016/s0378-5955(03)00222-3 [doi]
PST - ppublish
SO  - Hear Res. 2003 Sep;183(1-2):73-83. doi: 10.1016/s0378-5955(03)00222-3.