PMID- 13679305
OWN - NLM
STAT- MEDLINE
DCOM- 20040224
LR  - 20200930
IS  - 0363-6143 (Print)
IS  - 0363-6143 (Linking)
VI  - 286
IP  - 2
DP  - 2004 Feb
TI  - IgE alone-induced actin assembly modifies calcium signaling and degranulation in 
      RBL-2H3 mast cells.
PG  - C256-63
AB  - In the mast cell signaling pathways, the binding of immunoglobulin E (IgE) to 
      FcepsilonRI, its high-affinity receptor, is generally thought to be a passive 
      step. In this study, we examined the effect of IgE alone, that is, without 
      antigen stimulation, on the degranulation in mast cells. Monomeric IgE (500-5,000 
      ng/ml) alone increased cytosolic Ca2+ level ([Ca2+]i) and induced degranulation 
      in rat basophilic leukemia (RBL)-2H3 mast cells. Monomeric IgE (5,000 ng/ml) 
      alone also increased [Ca2+]i and induced degranulation in bone marrow-derived 
      mast cells. Interestingly, monomeric IgE (5-50 ng/ml) alone, in concentrations 
      too low to induce degranulation, increased filamentous actin content in RBL-2H3 
      mast cells. We next examined whether actin dynamics affect the IgE alone-induced 
      RBL-2H3 mast cell activation pathways. Cytochalasin D inhibited the ability of 
      IgE alone (50 ng/ml) to induce de novo actin assembly. In cytochalasin D-treated 
      cells, IgE (50 ng/ml) alone increased [Ca2+]i and induced degranulation. We have 
      summarized the current findings into two points. First, IgE alone increases 
      [Ca2+]i and induces degranulation in mast cells. Second, IgE, at concentrations 
      too low to increase either [Ca2+]i or degranulation, significantly induces actin 
      assembly, which serves as a negative feedback control in the mast cell Ca2+ 
      signaling and degranulation.
FAU - Oka, Tatsuya
AU  - Oka T
AD  - Department of Veterinary Pharmacology, Graduate School of Agriculture and Life 
      Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Hori, Masatoshi
AU  - Hori M
FAU - Tanaka, Akane
AU  - Tanaka A
FAU - Matsuda, Hiroshi
AU  - Matsuda H
FAU - Karaki, Hideaki
AU  - Karaki H
FAU - Ozaki, Hiroshi
AU  - Ozaki H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030917
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Actins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Actins/*drug effects/metabolism/*physiology
MH  - Animals
MH  - Calcium/metabolism
MH  - Calcium Signaling/*physiology
MH  - Cell Degranulation/drug effects/*physiology
MH  - Cell Line, Tumor
MH  - Immunoglobulin E/*pharmacology
MH  - Intracellular Membranes/metabolism
MH  - Mast Cells/drug effects/*physiology
MH  - Osmolar Concentration
MH  - Rats
EDAT- 2003/09/19 05:00
MHDA- 2004/02/26 05:00
CRDT- 2003/09/19 05:00
PHST- 2003/09/19 05:00 [pubmed]
PHST- 2004/02/26 05:00 [medline]
PHST- 2003/09/19 05:00 [entrez]
AID - 00197.2003 [pii]
AID - 10.1152/ajpcell.00197.2003 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2004 Feb;286(2):C256-63. doi: 
      10.1152/ajpcell.00197.2003. Epub 2003 Sep 17.