PMID- 13680850
OWN - NLM
STAT- MEDLINE
DCOM- 20040507
LR  - 20121115
IS  - 0269-3879 (Print)
IS  - 0269-3879 (Linking)
VI  - 17
IP  - 6
DP  - 2003 Sep
TI  - Determination of 3-n-butylphthalide in rabbit plasma by HPLC with fluorescence 
      detection and its application in pharmacokinetic study.
PG  - 391-5
AB  - A rapid, sensitive and specific reversed-phase high-performance liquid 
      chromatographic method was developed for the determination of 3-n-butylphthalide, 
      a drug currently being developed for treatment of stroke, in rabbit plasma. 
      Fluorescence detection at an excitation wavelength of 280 nm and an emission 
      wavelength of 304 nm was used for quantification of 3-n-butylphthalide. Ibuprofen 
      was used as internal standard. Plasma samples were extracted with diethyl ether 
      under acidic conditions. After evaporation of the organic phase, the extract was 
      dissolved in mobile phase and injected into the chromatograph with C(18) column 
      and a mobile phase of 0.05 mol/L sodium acetate buffer (pH 4.5)-acetonitrile 
      (400:600). The peak area ratio vs concentration in plasma was linear over the 
      range of 0.0212-4.24 microg/mL (correlation coefficient r = 0.9984) and the limit 
      of quantification was 0.0212 microg/mL. Mean recovery was determined as 101.0% by 
      analysis of plasma standard samples containing 0.0424, 0.424, 2.12 and 4.24 
      microg/mL of 3-n-butylphthalide. The intra-day relative standard deviations 
      (RSDs) ranged from 3.6 to 8.9% and inter-day RSDs were within 8.0%. 
      Pharmacokinetics of a single intravenous dose of 3-n-butylphthalide to the 
      rabbits was presented to illustrate the applicability of this method. 
      3-n-Butylphthalide exhibited linear pharmacokinetics after intravenous 
      administration to rabbits over the dose range 1-10 mg/kg.
CI  - Copyright 2003 John Wiley & Sons, Ltd.
FAU - Zhao, Chunshun
AU  - Zhao C
AD  - Department of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, 
      People's Republic of China.
FAU - He, Zhonggui
AU  - He Z
FAU - Cui, Shengmiao
AU  - Cui S
FAU - Zhang, Ruhua
AU  - Zhang R
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Aldehydes)
RN  - H21352682A (butyraldehyde)
SB  - IM
MH  - Aldehydes/*blood/pharmacokinetics
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Male
MH  - Rabbits
MH  - Spectrometry, Fluorescence/*methods
EDAT- 2003/09/19 05:00
MHDA- 2004/05/08 05:00
CRDT- 2003/09/19 05:00
PHST- 2003/09/19 05:00 [pubmed]
PHST- 2004/05/08 05:00 [medline]
PHST- 2003/09/19 05:00 [entrez]
AID - 10.1002/bmc.255 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2003 Sep;17(6):391-5. doi: 10.1002/bmc.255.