PMID- 1368316
OWN - NLM
STAT- MEDLINE
DCOM- 19920819
LR  - 20191028
IS  - 0167-7799 (Print)
IS  - 0167-7799 (Linking)
VI  - 10
IP  - 5
DP  - 1992 May
TI  - Message amplification phenotyping (MAPPing)--principles, practice and potential.
PG  - 146-52
AB  - Message amplification phenotyping (MAPPing) is a sensitive polymerase chain 
      reaction (PCR)-based technique for the rapid determination of the mRNA phenotype 
      of small numbers of cells. Isolated mRNA is reverse-transcribed (RT) into DNA, 
      and then the DNA fragments corresponding to proteins or genes of interest are 
      specifically amplified by PCR. The technique, also called RT-PCR, has wide 
      application in biology and medicine, and comparison with bioassays demonstrates 
      that MAPPing saves significant time and material. The technique should be 
      applicable to analyse mRNAs of virtually any tissue or cell type.
FAU - Larrick, J W
AU  - Larrick JW
AD  - Palo Alto Institute for Molecular Medicine, Stanford, CA 94309-8102.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Trends Biotechnol
JT  - Trends in biotechnology
JID - 8310903
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
MH  - Animals
MH  - Base Sequence
MH  - Biotechnology/trends
MH  - DNA/genetics
MH  - Gene Amplification
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phenotype
MH  - Polymerase Chain Reaction/*methods/trends
MH  - RNA, Messenger/*genetics
RF  - 33
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
AID - 10.1016/0167-7799(92)90202-7 [doi]
PST - ppublish
SO  - Trends Biotechnol. 1992 May;10(5):146-52. doi: 10.1016/0167-7799(92)90202-7.