PMID- 1368703
OWN - NLM
STAT- MEDLINE
DCOM- 19910930
LR  - 20061115
IS  - 0002-1369 (Print)
IS  - 0002-1369 (Linking)
VI  - 55
IP  - 6
DP  - 1991 Jun
TI  - Cloning and nucleotide sequences of the AccI restriction-modification genes in 
      Acinetobacter calcoaceticus.
PG  - 1553-9
AB  - The genes of the AccI restriction-modification system specific for GT(A/C) 
      (G/T)AC were cloned from the chromosomal DNA of Acinetobacter calcoaceticus, and 
      their nucleotides sequenced. The restriction and modification genes coded for 
      polypeptides with calculated molecular weights of 42,494 and 63,078, 
      respectively. Both the enzymes were coded by the same DNA strand and the 
      restriction gene was upstream of the methylase gene, separated by 2 bp. The 
      restriction gene was significantly expressed in E. coli cells, so that the AccI 
      restriction endonuclease could be purified to homogeneity. Analysis by sodium 
      dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration indicated 
      that the catalytically active form of the endonuclease was tetrameric. Sequence 
      comparison with related enzymes indicated that AccI methylase contained a segment 
      of tetra-amino acids, NPPY, characteristic of N6-adenine methylases. In addition, 
      some homologous regions were found in the sequence of HincII methylase specific 
      for GT(C/T) (A/G)AC.
FAU - Kawakami, B
AU  - Kawakami B
AD  - Tsuruga Enzyme Plant, Toyobo Co., Ltd., Fukui, Japan.
FAU - Christophe, H
AU  - Christophe H
FAU - Nagatomo, M
AU  - Nagatomo M
FAU - Oka, M
AU  - Oka M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - Agric Biol Chem
JT  - Agricultural and biological chemistry
JID - 0370452
RN  - 0 (DNA Restriction-Modification Enzymes)
MH  - Acinetobacter/enzymology/*genetics
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA Restriction-Modification Enzymes/biosynthesis/*genetics
MH  - Escherichia coli/genetics/metabolism
MH  - Gene Expression
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Sequence Homology, Nucleic Acid
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
PST - ppublish
SO  - Agric Biol Chem. 1991 Jun;55(6):1553-9.